Effect of pH on isolation and distribution of members of subdivision 1 of the phylum Acidobacteria occurring in soil (2006)

Content Provider	CiteSeerX
Author	Sait, Michelle Davis, Kathryn E. R. Janssen, Peter H.
Abstract	The pH strongly influenced the development of colonies by members of subdivision 1 of the phylum Acidobacteria on solid laboratory media. Significantly more colonies of this group formed at pH 5.5 than at pH 7.0. At pH 5.5, 7 to 8 % of colonies that formed on plates that were incubated for 4 months were formed by subdivision 1 acidobacteria. These colonies were formed by bacteria that spanned almost the entire phylogenetic breadth of the subdivision, and there was considerable congruence between the diversity of this group as determined by the cultivation-based method and by surveying 16S rRNA genes in the same soil. Members of subdivision 1 acidobacteria therefore appear to be readily culturable. An analysis of published libraries of 16S rRNAs or 16S rRNA genes showed a very strong correlation between the abundance of subdivision 1 acidobacteria in soil bacterial communities and the soil pH. Subdivision 1 acidobacteria were most abundant in libraries from soils with pHs of <6, but rare or absent in libraries from soils with pHs of>6.5. This, together with the selective cultivation of members of the group on lower-pH media, indicates that growth of many members of subdivision 1 acidobacteria is favored by slightly to moderately acidic growth conditions. Members of subdivision 1 of the phylum Acidobacteria (29) are widely distributed in soils and other habitats (2, 3, 5, 17, 19,
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Publisher Date	2006-01-01
Access Restriction	Open
Subject Keyword	Phylum Acidobacteria Rrna Gene Soil Ph Considerable Congruence Strong Correlation Lower-ph Medium Entire Phylogenetic Breadth Soil Bacterial Community Selective Cultivation Cultivation-based Method Solid Laboratory Medium Acidic Growth Condition Published Library Many Member
Content Type	Text