NDLI: Human retinal pigment epithelial lysis of extracellular matrix: functional urokinase plasminogen activator receptor, collagenase, and elastase.

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Human retinal pigment epithelial lysis of extracellular matrix: functional urokinase plasminogen activator receptor, collagenase, and elastase.

Content Provider	CiteSeerX
Author	Elner, G.
Abstract	Purpose: To show (1) human retinal pigment epithelial (HRPE) expression of functional urokinase plasminogen acti-vator receptor (uPAR; CD87), (2) HRPE secretion of collagenase and elastase, (3) uPAR-dependent HRPE migration, and (4) uPAR expression in diseased human retinal tissue. Methods: Immunohistochemistry for uPAR was performed on cultured HRPE cells and in sections of human retina. Double-immunofluorescent staining of live human RPE cells with anti-CR3 antibody (CD11b) was performed to demonstrate the physical proximity of this ß2 integrin with uPAR and determine whether associations were dependent on RPE confluence and polarity. Extracellular proteolysis by HRPE uPAR was evaluated using fluorescent bodipy-BSA and assessed for specificity by plasminogen activator inhibitor-1 (PAI-1) inhibition. The effect of interleukin-1ß (IL-1ß) on uPAR expression was assessed. Collagenase and elastase secretion by unstimulated and IL-1–stimulated HRPE cells was measured by 3H-labelled collagen and elastin cleavage. HRPE-associated collagenase was also assessed by cleavage of fluorescent DQ-collagen and inhibited by phenanthroline. Using an extracellular matrix assay, the roles of uPAR and collagenase in HRPE migration were assessed. Results: Immunoreactive uPAR was detected on cultured HRPE cells and increased by IL-1. On elongated, live HRPE cells, uPAR dissociated from CD11b (CR3) and translocated to anterior poles of migrating cells. Extracellular proteoly-
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Subject Keyword	Functional Urokinase Plasminogen Activator Receptor Upar Expression Cultured Hrpe Cell Hrpe Cell Fluorescent Bodipy-bsa Rpe Confluence 3h-labelled Collagen Hrpe-associated Collagenase Human Retinal Pigment Epithelial Anti-cr3 Antibody Extracellular Proteolysis Live Human Rpe Cell Hrpe Upar Human Retina Plasminogen Activator Immunoreactive Upar Extracellular Matrix Assay Hrpe Secretion Diseased Human Retinal Tissue Extracellular Proteoly Functional Urokinase Plasminogen Acti-vator Receptor Live Hrpe Cell Physical Proximity Hrpe Migration Fluorescent Dq-collagen Upar-dependent Hrpe Migration Double-immunofluorescent Staining Elastin Cleavage
Content Type	Text

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