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Resazurin metabolism assay is a new sensitive alternative test in isolated pig cornea (2002).
| Content Provider | CiteSeerX |
|---|---|
| Author | Martin, Chantal Rat, Patrice Warnet, Jean-Michel |
| Abstract | The main object of our study was to investigate whether the resazurin metabolism assay is a sensitive surfactant and alcohol toxicity test in isolated pig cornea and to compare this recently developed fluorometric assay with the data collected in the eye irritation reference chemical data bank. Resazurin is a substrate that changes color in response to metabolic activity. Isolated pig corneas were immersed for 10 min in surfactants and alcohol irritant solutions. After incubation, resorufin fluorescence was read and corneal viability was assessed. This corneal viability was compared with the maximal modified average score published in the report of ECETOC. This assay highlighted different concen-tration-dependent irritation potentials of the three surfactants tested, and the same results were obtained with corneas treated with the alcohols. We observed that the degree of surfactant- and alcohol-induced decrease in corneal viability, using the resazurin reduction test, was correlated with the in vivo irritancy measure-ments as determined by the Draize test and scored with the Modified Maximum Average Score (MMAS). This assay allowed us to classify the ocular irritancy of the tested surfactants and alcohols in the same ranking order as the Draize classification. Corneal viability measurement can be used as a potential alterna-tive for the toxicological assessment of surfactants and alcohols. The nontoxic, nonradioactive resazurin metabolism assay allows rapid assessment of many samples with simple equipment and at reduced cost for continuous monitoring of corneal viability. This assay seems to be suitable as a toxicological screening test for eye irritation determination. Key Words: isolated corneal model; toxicological assessment; resazurin; surfactants; alcohols. |
| File Format | |
| Publisher Date | 2002-01-01 |
| Access Restriction | Open |
| Subject Keyword | Corneal Viability Isolated Pig Cornea Resazurin Metabolism Assay New Sensitive Alternative Test Toxicological Assessment Resazurin Reduction Test Modified Maximum Average Score Vivo Irritancy Measure-ments Alcohol Irritant Solution Corneal Viability Measurement Maximal Modified Average Score Many Sample Resorufin Fluorescence Draize Classification Potential Alterna-tive Main Object Draize Test Toxicological Screening Test Isolated Corneal Model Continuous Monitoring Ranking Order Nonradioactive Resazurin Metabolism Alcohol-induced Decrease Pig Cornea Metabolic Activity Rapid Assessment Tested Surfactant Different Concen-tration-dependent Irritation Potential Simple Equipment Ocular Irritancy Sensitive Surfactant Fluorometric Assay Alcohol Toxicity Test Eye Irritation Determination |
| Content Type | Text |
