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Distribution and lateral mobility of glycine receptors on cultured spinal cord neurons.
| Content Provider | CiteSeerX |
|---|---|
| Author | Srinivasan, Yogambal Guzikowski, Anthony P. Haugl, Richard P. Angelides, Kimon J. |
| Abstract | Strychnine is one of the most potent antagonists of glycine-mediated inhibitory conductances in the mammalian spinal cord. In order to examine the distribution of glycine receptors (GlyRs) on neuronal cells, 2 novel fluorescent strychnine derivatives were synthesized and characterized chemically, spectroscopically, and biologically. Both compounds retain their biological activity after derivatization and are potent inhibitors of 3H-strychnine binding to bovine spinal cord membranes and membranes from rat spinal cord cultures. Using these fluorescent strychnine analogs, the cellular dis-tribution and lateral mobility of GlyRs on cultured rat spinal cord neurons were studied by digital fluorescence imaging and photobleach recovery microscopy. On these neurons, even in the absence of observable synaptic contact and early in development GlyRs are predominantly localized to |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Glycine Receptor Lateral Mobility Cultured Spinal Cord Neuron Cellular Dis-tribution Biological Activity Spinal Cord Membrane Observable Synaptic Contact Potent Inhibitor 3h-strychnine Binding Novel Fluorescent Strychnine Derivative Glycine-mediated Inhibitory Conductance Photobleach Recovery Microscopy Rat Spinal Cord Culture Fluorescent Strychnine Analog Development Glyrs Digital Fluorescence Imaging Neuronal Cell Potent Antagonist Mammalian Spinal Cord Cultured Rat Spinal Cord Neuron |
| Content Type | Text |
