NDLI: Review labeling antigen-specific cd4+ t cells with class ii mhc oligomers (2001).

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Review labeling antigen-specific cd4+ t cells with class ii mhc oligomers (2001).

Content Provider	CiteSeerX
Author	Thomas O. Cameron, A. Philip J. Norris, B. Alka Patel, C. Corinne Moulon, D. Eric S. Rosenberg, B.
Abstract	Class I MHC–peptide oligomers (MHC tetramers) have become popular reagents for the detection and characterization of antigen-specific CD8 + T cells. Class II MHC proteins can be produced by expression in Escherichia coli followed by in vitro folding, or by native expression in insect cells; biotin can be introduced by site-specific chemical modification of cysteine, or by enzymatic modification of a peptide tag; and a variety of fluorescent streptavidin preparations can be used for oligomerization. Here we review methodologies for production of fluorescent oligomers of soluble class II MHC proteins and discuss their use in analysis of antigen-specific CD4 + T cells. We explore the experimental conditions necessary for efficient staining of CD4 + T cells using oligomers of class II MHC proteins, and we establish a standard protocol. Finally, we consider complications and challenges associated with these reagents, discuss the interpretation of staining results, and suggest future directions for investigation, in particular the use of MHC oligomers for the study of T cell avidity modulation.
File Format	PDF
Publisher Date	2001-01-01
Access Restriction	Open
Subject Keyword	Review Labeling Antigen-specific Cd4 Cell Class Ii Mhc Oligomers Class Ii Mhc Protein Enzymatic Modification Peptide Tag Experimental Condition Cd4 Cell Cell Avidity Modulation Site-specific Chemical Modification Native Expression Standard Protocol Mhc Peptide Oligomers Mhc Tetramers Soluble Class Ii Mhc Protein Fluorescent Oligomers Efficient Staining Escherichia Coli Fluorescent Streptavidin Preparation Insect Cell Future Direction Vitro Folding Mhc Oligomers Popular Reagent Antigen-specific Cd8 Cell Antigen-specific Cd4 Cell
Content Type	Text

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