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Modulation of ADAR1 editing activity by Z-RNA in vitro (2005)
| Content Provider | CiteSeerX |
|---|---|
| Author | Koeris, Michael Funke, Lars Shrestha, Jay Rich, Er Maas, Stefan |
| Abstract | RNA editing by A-to-I modification has been recog-nized as an important molecular mechanism for gen-erating RNA and protein diversity. In mammals, it is mediated by a family of adenosine deaminases that act on RNAs (ADARs). The large version of the editing enzyme ADAR1 (ADAR1-L), expressed from an interferon-responsible promoter, has a Z-DNA/Z-RNA binding domain at its N-terminus. We have tested the in vitro ability of the enzyme to act on a 50 bp segment of dsRNA with or without a Z-RNA forming nucleotide sequence. A-to-I editing efficiency is mark-edly enhanced in presence of the sequence favoring Z-RNA. In addition, an alteration in thepattern of modi-fication along the RNA duplex becomes evident as reaction times decrease. These results suggest that the local conformation of dsRNA molecules might be an important feature for target selectivity by ADAR1 and other proteins with Z-RNA binding domains. |
| File Format | |
| Journal | Nucleic Acids Res |
| Language | English |
| Publisher Date | 2005-01-01 |
| Access Restriction | Open |
| Subject Keyword | Protein Diversity Important Feature Target Selectivity Vitro Ability Large Version Z-dna Z-rna Binding Domain A-to-i Modification Bp Segment Gen-erating Rna Z-rna Forming Nucleotide Sequence Dsrna Molecule A-to-i Editing Efficiency Reaction Time Interferon-responsible Promoter Rna Duplex Local Conformation Important Molecular Mechanism Enzyme Adar1 Adenosine Deaminases Z-rna Binding Domain |
| Content Type | Text |
| Resource Type | Article |
