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Effect of mutations within the cys-rich region of potyvirus helper component-proteinase on self-interaction.
| Content Provider | CiteSeerX |
|---|---|
| Author | Urcuqui-Inchima, Silvio Maia, Ivan G. Drugeon, Gabrie Le Haenni, Anne-Lise |
| Abstract | The first C60 amino acids of the N-terminal part of the potyvirus helper component-proteinase (HC-Pro) include highly conserved residues comprising a Cys-rich region. In the present study, the domain in Potato virus Y sufficient for self-interaction was mapped using the yeast two-hybrid system to the 83 N-terminal amino acids of HC-Pro. Mutations in the conserved His and two Cys residues within the Cys-rich region have a strong debilitating effect on self-interaction when introduced in the full-length HC-Pro, but not when introduced in the N-terminal fragment. The genome of Potato virus Y (PVY), the type member of the genus Potyvirus, contains a monopartite single-stranded RNA of positive polarity. It is translated into a single |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Cys-rich Region Potyvirus Helper Component-proteinase Genus Potyvirus Potato Virus Sufficient Strong Debilitating Effect Present Study Conserved Residue Positive Polarity Cys Residue Full-length Hc-pro N-terminal Amino Acid N-terminal Fragment Type Member Monopartite Single-stranded Rna Two-hybrid System First C60 Amino Acid N-terminal Part Potato Virus |
| Content Type | Text |
