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Methanol-associated matrix effects in electrospray ionization tandem mass spectrometry
| Content Provider | CiteSeerX |
|---|---|
| Author | Annesley, Thomas M. |
| Abstract | Background: Matrix effects can profoundly reduce the performance of electrospray ionization mass spectrom-etry. Preliminary observations indicated that the meth-anol used in the mobile phase could be a source of differential ionization or ion suppression. Methods: Drug stability studies, analysis of biological extracts, mixing experiments, and postcolumn infusions were used to test 9 commercial methanols for ionization differences in liquid chromatography-tandem mass spectrometry assays for immunosuppressants. Area re-sponses for the drugs and internal standards were compared for mobile phases prepared with each se-lected methanol. Postcolumn infusion experiments were performed to confirm the degree of ionization differ-ences occurring at the ion source, and to evaluate the |
| File Format | |
| Journal | Clin. Chem |
| Access Restriction | Open |
| Subject Keyword | Se-lected Methanol Methanol-associated Matrix Effect Internal Standard Preliminary Observation Postcolumn Infusion Ionization Difference Postcolumn Infusion Experiment Ion Source Ion Suppression Drug Stability Study Biological Extract Differential Ionization Ionization Differ-ences Electrospray Ionization Tandem Mass Spectrometry Matrix Effect Mobile Phase Commercial Methanol Area Re-sponses Liquid Chromatography-tandem Mass Spectrometry Assay Electrospray Ionization Mass Spectrom-etry |
| Content Type | Text |