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Preparative isoelectric focusing of poliovirus polypeptides in urea-sucrose gradients.
| Content Provider | CiteSeerX |
|---|---|
| Author | Wiegers, K. J. Drzeniek, R. |
| Abstract | Isoelectric focusing in urea using small density gradient columns for dissociated poliovirus resulted in a complete separation of the four virus polypeptides. Identification and purity of VPI, 2, 3 and 4 was shown by SDS-polyacrylamide g l electrophoresis. The isoelectric points were determined and compared with the values obtained in gels. The recovery of the individual polypeptides was about 8o %. Up to 5oo #g of poliovirus per tube could thus be separated in a one-step ro-cedure giving pure and SDS-free poliovirus polypeptides. |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Preparative Isoelectric Focusing Urea-sucrose Gradient Poliovirus Polypeptide Isoelectric Focusing Sds-polyacrylamide Electrophoresis Complete Separation Isoelectric Point Dissociated Poliovirus Small Density Gradient Column Virus Polypeptide Individual Polypeptide Sds-free Poliovirus Polypeptide One-step Ro-cedure |
| Content Type | Text |