Methylmercury Measurement in Whole Blood by Isotope-Dilution GC-ICPMS with 2 Sample Preparation Methods

Content Provider	CiteSeerX
Author	Baxter, Douglas C. Klockare, Dennis Rodushkin, Ilia Waara, Hans
Abstract	cury is rarely measured directly in clinical studies; instead, conclusions are based on total mercury mea-surements. We have developed isotope–dilution-based methods for methylmercury-specific analysis of whole blood by coupled gas chromatography–inductively cou-pled plasma mass spectrometry (GC-ICPMS). Methods: We analyzed animal and human blood sam-ples after alkaline digestion or extraction of methylmer-cury into dichloromethane and back extraction into water. Methylmercury was converted to the volatile ethyl derivative, purged, and trapped on a solid-phase collection medium, and then introduced into the GC-ICPMS system. Results: Limits of quantification were 0.4 and 0.03 g/L at a signal-to-noise ratio of 10 with the alkaline diges-
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Subject Keyword	Sample Preparation Method Methylmercury Measurement Cou-pled Plasma Mass Spectrometry Alkaline Diges Solid-phase Collection Medium Whole Blood Gc-icpms System Alkaline Digestion Human Blood Sam-ples Signal-to-noise Ratio Total Mercury Mea-surements Volatile Ethyl Derivative Isotope-dilution Gc-icpms Gas Chromatography Back Extraction Dilution-based Method Clinical Study Methylmercury-specific Analysis
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