NDLI: Endovascular Treatment of Experimental Aneurysms With Use of Fibroblast Transfected With Replication-Deficient Adenovirus Containing

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Endovascular Treatment of Experimental Aneurysms With Use of Fibroblast Transfected With Replication-Deficient Adenovirus Containing

Content Provider	CiteSeerX
Author	Cloft, H. J. Helm, G. A. Gene, Protein- Dai, D. Lewis, D. A. Morphogenetic, Bone Ding, Y. H. Kallmes, D. F. Danielson, M. A. Kadirvel, R.
Abstract	BACKGROUND AND PURPOSE: Modified coils have failed to improve long-term recanalization of aneu-rysms. This study examined whether ex vivo transduction of replication-deficient adenovirus contain-ing the bone morphogenetic protein-13 gene (Ad-BMP-13) in fibroblast allografts would improve angiographic results via increased collagen synthesis, compared with fibroblast-coated platinum coils (FBC) and bare platinum coils (PA). MATERIALS AND METHODS: Aneurysms were embolized with Ad-BMP-13-coated coils (n 20). Rabbits were sacrificed at 14 days and at 1, 3, and 6 months after implantation. Digital subtraction angiography (DSA) evaluated stability after embolization. Histologic specimens were examined with a qualitative grading system. Masson trichrome evaluated collagen deposition. Findings were compared with previously reported controls for PA and FBC in the same model and time points. RESULTS: The grading system showed a greater total score (P .0002) in Ad-BMP-13 (6.8 1.6) and FBC (6.3 2.4) compared with PA (4.7 2.4). A group main effects test showed that aneurysm neck tissue coverage in Ad-BMP-13 (2.5 1.1) was higher (P .0007) than both FBC (1.6 1.4) and PA (0.9 1.1). Ad-BMP-13 had more (P .0001) collagen deposition than the FBC and PA. One- and 3-month Ad-BMP-13 collagen depositions increased (P .05) over the FBC and PA. Finally, Ad-BMP-13
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Subject Keyword	Bone Morphogenetic Protein-13 Gene Fibroblast Transfected Total Score Long-term Recanalization Fibroblast Allograft Group Main Effect Test Time Point Ad-bmp-13-coated Coil Bare Platinum Coil Grading System Endovascular Treatment Collagen Deposition Histologic Specimen Aneurysm Neck Tissue Coverage Digital Subtraction Angiography Masson Trichrome Experimental Aneurysm Replication-deficient Adenovirus Fibroblast-coated Platinum Coil Increased Collagen Synthesis Replication-deficient Adenovirus Containing 3-month Ad-bmp-13 Collagen Deposition Angiographic Result Qualitative Grading System
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