NDLI: Tympanic membrane changes in experimental acute otitis media and myringotomy.

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Tympanic membrane changes in experimental acute otitis media and myringotomy.

Content Provider	CiteSeerX
Author	Alzbutienė, Giedrė Hermansson, Ann Cayè-Thomasen, Per Kinduris, Vytenis
Abstract	Key words: acute otitis media; myringotomy; myringosclerosis; animal model. Summary. Objective. The present experimental study explored pathomorphological changes and calcium depositions in the tympanic membrane during experimental acute otitis media caused by nontypeable Haemophilus influenzae in myringotomized and nonmyringotomized ears. Material and methods. A rat model of experimental acute otitis media caused by nontypeable Haemophilus influenzae was employed. Sixteen Sprague-Dawley rats were used. Four days following middle ear inoculation, a bilateral myringotomy was performed in six randomly selected animals. Another group of 10 animals was inoculated only. On days 4, 7, 14, and 28 after inoculation, two animals from each group were sacrificed. The temporal bones were removed and the tympanic membranes were dissected, followed by paraffin embedding. Adjacent sections were stained with PAS-alcian blue for basic histopathological observations and by von Kossa method for determination of calcium phosphate depositions. Results. Particularly intense invasion of polymorphonuclear neutrophil leukocytes was seen on day 4 after inoculation. The highest infiltration of macrophages was observed on day 7. The peak number of lymphocytes was seen on day 14. No difference occurred in the number of
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Subject Keyword	Experimental Acute Otitis Medium Tympanic Membrane Change Tympanic Membrane Nontypeable Haemophilus Influenzae Nonmyringotomized Ear Polymorphonuclear Neutrophil Leukocyte Pathomorphological Change Calcium Deposition Pas-alcian Blue Adjacent Section Middle Ear Inoculation Present Experimental Study Basic Histopathological Observation Rat Model Peak Number Von Kossa Method Animal Model Temporal Bone Bilateral Myringotomy Calcium Phosphate Deposition Particularly Intense Invasion Sixteen Sprague-dawley Rat Key Word Acute Otitis Medium
Content Type	Text

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