NDLI: Efficacy of sirna nanocapsules targeted against the ews–fli1 (2005).

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Efficacy of sirna nanocapsules targeted against the ews–fli1 (2005).

Content Provider	CiteSeerX
Author	Toub, Nedjma Bertr, Jean-Rémi Tamaddon, Ali Elhamess, Hind Hillaireau, Hervé Maksimenko, Andrei Maccario, Jean Malvy, Claude Fattal, Elias Couvreur, Patrick
Abstract	Abstract. The EWSYFli1 fusion gene encodes for a chimeric oncogenic transcription factor considered to be the cause of the Ewing sarcoma. The efficiency of small interfering RNAs (siRNAs) targeted toward the EWSYFli1 transcript (at the junction point type 1) was studied, free or encapsulated into recently developed polyisobutylcyanoacrylate aqueous core nanocapsules. Because this mRNA sequence is only present in cancer cells, it therefore constituted a relevant target. Studies of the intracellular penetration by confocal microscopy in NIH/3T3 EWSYFli1 cells showed that nanocapsules improved the intracellular penetration of siRNA with mainly a cytoplasmic localization. These biodegradable siRNA-loaded nanocapsules were then tested in vivo on a mice xenografted EWSYFli1-expressing tumor; they were found to trigger a dose-dependant inhibition of tumor growth after intratumoral injection. A specific inhibition of EWSYFli1 was observed, too. These findings now open new prospects for the treatment of experimental cancers with junction oncogenes. KEY WORDS: Ewing sarcoma; EWSYFli1; isobutylcyanoacrylate; nanocapsules; siRNA.
File Format	PDF
Publisher Date	2005-01-01
Access Restriction	Open
Subject Keyword	Sirna Nanocapsules Targeted Ew Fli1 Intracellular Penetration Ewing Sarcoma Ewsyfli1 Transcript Confocal Microscopy Chimeric Oncogenic Transcription Factor Experimental Cancer Cytoplasmic Localization Polyisobutylcyanoacrylate Aqueous Core Nanocapsules Specific Inhibition Dose-dependant Inhibition Junction Oncogene Biodegradable Sirna-loaded Nanocapsules New Prospect Ewsyfli1 Fusion Gene Encodes Intratumoral Injection Junction Point Type Ewsyfli1-expressing Tumor Cancer Cell Mrna Sequence Ewsyfli1 Cell Relevant Target Key Word Tumor Growth
Content Type	Text
Resource Type	Article

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