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Kynurenic acid enhances expression of p21 waf1/cip1 in colon cancer ht-29 cells.
| Content Provider | CiteSeerX |
|---|---|
| Author | Walczak, Katarzyna Turski, Waldemar A. Rzeski, Wojciech |
| Abstract | Background: Kynurenic acid (KYNA), a tryptophan metabolite, was found in the mucus of rat small intestine. However, its role in the gastrointestinal tract is still not fully elucidated. Methods: To verify whether KYNA affects cell cycle regulators, the protein expression of cyclin-dependent kinase inhibitor p21 Waf1/Cip1 was investigated in colon adenocarcinoma HT-29 cells exposed to KYNA. MTT, BrdU assay and siRNA technology were used to evaluate the effect of KYNA on cancer cell proliferation. Results: KYNA significantly enhanced the expression of p21 Waf1/Cip1. Importantly, the overexpression of this protein was involved in inhibition of proliferation and DNA synthesis in HT-29 cells. Conclusions: KYNA may be considered as a potential chemoprevention agent against colon cancer. Key words: kynurenic acid, colon cancer, p21 Waf1/Cip1, proliferation |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Kynurenic Acid P21 Waf1 Cip1 Colon Cancer Ht-29 Cell Colon Cancer Sirna Technology Cancer Cell Proliferation Dna Synthesis Waf1 Cip1 Protein Expression Ht-29 Cell Tryptophan Metabolite Key Word Brdu Assay Cell Cycle Regulator Potential Chemoprevention Agent Gastrointestinal Tract Colon Adenocarcinoma Ht-29 Cell Rat Small Intestine |
| Content Type | Text |