NDLI: Itraq isobaric tags for relative and absolute (2010).

Content Provider	CiteSeerX
Author	Turck, Christoph W. Martins-De-Souza, Daniel Turck, G. Maccarrone C. W. Martins-De-Souza, D.
Abstract	Ó The Author(s) 2010. This article is published with open access at Springerlink.com Abstract We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow we used the ‘Isotope-Coded Protein Labeling ’ (ICPL) method for accurate relative quantitation of protein expression. Such workflow was successfully applied to a comparative proteome analysis of schizophrenia versus healthy control brain tissues and can be an alternative to proteome researches.
File Format	PDF
Publisher Date	2010-01-01
Access Restriction	Open
Subject Keyword	Itraq Isobaric Tag Isoelectric Focusing High Throughput Mass Spectrometry Workflow Protein Expression Healthy Control Brain Tissue Isotope-coded Protein Labeling Bidimensional Peptide Fractionation Procedure Open Access Comparative Proteome Analysis Spectrometric Analysis Peptide Separation Accurate Relative Quantitation Protein Identification
Content Type	Text
Resource Type	Article

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