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An FFT-based Method for Attenuation Correction in Fluorescence Confocal Microscopy (1993)
| Content Provider | CiteSeerX |
|---|---|
| Author | Roerdink Roerdink, J. B. T. M. Bakker, M. |
| Abstract | this paper we propose a new method to correct for these effects. Our approach, valid for weak attenuation, consists in multiplying the measured fluorescent intensity by a correction factor involving a convolution integral of the measured signal, which can be computed efficiently by the Fast Fourier Transform. We give analytical and numerical estimates as to the degree of attenuation under which our method is valid, and apply the method to various test images. We also show a restoration of a real CSLM image. Finally the method is compared with a recent iterative method with regard to numerical accuracy and computational efficiency. AMS 1991 Mathematics Subject Classification: 68U10, 78A99. Keywords: Fluorescence confocal microscopy, attenuation correction, convolution method, Fast Fourier Transform, 3D image processing. Note: Postscript version obtainable at http://www.cs.rug.nl/ |
| File Format | |
| Volume Number | 169 |
| Journal | J. Microscopy |
| Language | English |
| Publisher Date | 1993-01-01 |
| Access Restriction | Open |
| Subject Keyword | Attenuation Correction Fluorescence Confocal Microscopy Fft-based Method Fast Fourier Transform Weak Attenuation Correction Factor Postscript Version Obtainable Numerical Estimate New Method Mathematics Subject Classification Numerical Accuracy Measured Signal Convolution Method Various Test Image Convolution Integral Real Cslm Image Recent Iterative Method Fluorescent Intensity Computational Efficiency Image Processing |
| Content Type | Text |
| Resource Type | Article |
