Ionotropic glutamate receptor ampa 1 is associated with ovulation rate (2010).

Content Provider	CiteSeerX
Author	Sugimoto, Mayumi Sasaki, Shinji Watanabe, Toshio Nishimura, Shota Ideta, Atsushi Matsuda, Keiko Yuzaki, Michisuke Sakimura, Kenji Aoyagi, Yoshito
Abstract	Ionotropic glutamate receptors mediate most excitatory neurotransmission in the central nervous system by opening ion channels upon the binding of glutamate. Despite the essential roles of glutamate in the control of reproduction and anterior pituitary hormone secretion, there is a limited understanding of how glutamate receptors control ovulation. Here we reveal the function of the ionotropic glutamate receptor AMPA-1 (GRIA1) in ovulation. Based on a genome-wide association study in Bos taurus, we found that ovulation rate is influenced by a variation in the N-terminal leucine/ isoleucine/valine-binding protein (LIVBP) domain of GRIA1, in which serine is replaced by asparagine. GRIA1 Asn has a weaker affinity to glutamate than GRIA1 Ser, both in Xenopus oocytes and in the membrane fraction of bovine brain. This single amino acid substitution leads to the decreased release of gonadotropin-releasing hormone (GnRH) in immortalized hypothalamic GT1-7 cells. Cows with GRIA1 Asn have a slower luteinizing hormone (LH) surge than cows with GRIA1 Ser.In addition, cows with GRIA1 Asn possess fewer immature ovarian follicles before superovulation and have a lower response to hormone treatment than cows with GRIA1 Ser. Our work identified that GRIA1 is a critical mediator of ovulation and that GRIA1 might be a useful target for reproductive therapy.
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Publisher Date	2010-01-01
Access Restriction	Open
Subject Keyword	Ovulation Rate Ionotropic Glutamate Receptor Ampa Gria1 Ser Gria1 Asn N-terminal Leucine Isoleucine Valine-binding Protein Critical Mediator Gonadotropin-releasing Hormone Gria1 Asn Posse Limited Understanding Membrane Fraction Ionotropic Glutamate Receptor Decreased Release Genome-wide Association Study Immortalized Hypothalamic Gt1-7 Cell Essential Role Ion Channel Anterior Pituitary Hormone Secretion Ionotropic Glutamate Receptor Ampa-1 Central Nervous System Useful Target Bovine Brain Immature Ovarian Follicle Single Amino Acid Substitution Xenopus Oocyte Bos Taurus Reproductive Therapy Excitatory Neurotransmission Glutamate Receptor
Content Type	Text