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Interchromatid and Interhomolog Recombination in Arabidopsis thaliana
| Content Provider | CiteSeerX |
|---|---|
| Author | Ries, Gerhard Barbara Hohn, A. Bonhoeffer, B. Sebastian Molinier, Jean |
| Abstract | Intermolecular recombination events were monitored in Arabidopsis thaliana lines using specially designed recombination traps consisting of tandem disrupted b-glucuronidase or luciferase reporter genes in direct repeat orientation. Recombination frequencies (RFs) varied between the different lines, indicating possible position effects influencing intermolecular recombination processes. The RFs between sister chromatids and between homologous chromosomes were measured in plants either hemizygous or homozygous for a transgene locus. The RFs in homozygous plants exceeded those of hemizygous plants by a factor of>2, implying that in somatic plant cells both sister chromatid recombination and recombination between homologous chromosomes exist for recombinational DNA repair. In addition, different DNA-damaging agents stimulated recombination in homozygous and hemizygous plants to different extents in a manner dependent on the type of DNA damage and on the genomic region. The genetic and molecular analysis of recombination events showed that most of the somatic recombination events result from gene conversion, although a pop-out event has also been characterized. |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Luciferase Reporter Gene Somatic Plant Cell Interhomolog Recombination Dna Damage Intermolecular Recombination Process Different Extent Recombination Event Somatic Recombination Event Homozygous Plant Different Dna-damaging Agent Arabidopsis Thaliana Genomic Region Hemizygous Plant Transgene Locus Different Line Intermolecular Recombination Event Arabidopsis Thaliana Line Manner Dependent Recombination Frequency Gene Conversion Recombinational Dna Repair Chromatid Recombination Direct Repeat Orientation Homologous Chromosome Recombination Trap Sister Chromatid Pop-out Event Possible Position Effect Molecular Analysis |
| Content Type | Text |