Loading...
Please wait, while we are loading the content...
Similar Documents
Gene expression of basic helix-loop-helix transcription factor, SHARP-2, is regulated by gonadotropins in the rat ovary and MA-10 cells (2004)
| Content Provider | CiteSeerX |
|---|---|
| Author | Yamada, Kazuya Kawata, Hiroko Mizutani, Tetsuya Arima, Takeshi Yazawa, Takashi Matsuura, Kaoru Shou, Zhangfei Sekiguchi, Toshio Yoshino, Miki Kajitani, Takashi |
| Abstract | Basic helix-loop-helix (bHLH) proteins regulate transcription from the E box sequence (5�-CANNTG-3�) located in the regulatory region of most gene promoters. The rat enhancer of split- and hairy-related protein 2 (SHARP-2) is a member of the bHLH protein family. To analyze the possible role of SHARP-2 in the rat ovary, the regulation of the expression of the SHARP-2 gene was examined, and the SHARP-2 protein was characterized. Northern blot analysis revealed that the level of SHARP-2 mRNA abruptly and temporarily increases as the result of the action of LH, i.e., eCG or hCG treatment alone or hCG after eCG treatment, in the rat ovary, as indicated by the treatment of primary cultured rat granulosa cells with hCG after FSH treatment or of mouse Leydig MA-10 cells with hCG or 8-bromoadenosine 3�,5�-cyclic monophosphate. An in situ |
| File Format | |
| Journal | Biol. Reprod |
| Language | English |
| Publisher Date | 2004-01-01 |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
