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The involvement of active dna synthesis in camptothecin-induced g2 arrest: altered regulation of p34cdc2/cyclin b1 (2013).
| Content Provider | CiteSeerX |
|---|---|
| Author | Cyclin, B. Tsao, Yeou-Ping Liu, Leroy F. |
| Abstract | Cell cycle arrest in G2 phase is a common response to a variety of DNA-damaging agents. The coupling between DNA damage and <. arrest was studied in synchronized HeLa cells using camptothecin, a highly specific inhibitor of topoisomerase I that damages DNA through the formation of reversible topoisomerase I-DNA cleavable complexes. Brief camptothecin treatment of early S-phase Urla cells caused arrest at <•: phase and abolished the activation of p,"M"'1 ' protein kinase. Both tyrosine dephosphorylation of p34cdc!and cyclin B accumulation were altered. These cell cycle-dependent changes were not observed when DNA replication was inhibited by aphidicolin during the brief campto thecin treatment. Our results suggest that to produce G2 arrest, active DNA synthesis is required at the time of camptothecin treatment, as was previously shown for camptothecin-induced cytotoxicity. Furthermore, our results suggest that the interaction of the replication fork with DNA damage may ultimately trigger altered regulation of p.M"1 ' V\clin B, leading to cell cycle arrest at the <.• ¿ phase. |
| File Format | |
| Publisher Date | 2013-01-01 |
| Access Restriction | Open |
| Subject Keyword | Active Dna Synthesis Altered Regulation P34cdc2 Cyclin B1 Camptothecin-induced G2 Arrest Camptothecin Treatment Brief Campto Thecin Treatment Cell Cycle Arrest Cyclin Accumulation Damage Dna Replication Fork Cycle Arrest Brief Camptothecin Treatment Tyrosine Dephosphorylation Specific Inhibitor Reversible Topoisomerase I-dna Cleavable Complex Synchronized Hela Cell Protein Kinase Common Response Cycle-dependent Change Dna-damaging Agent Early S-phase Urla Cell G2 Phase Dna Replication Camptothecin-induced Cytotoxicity |
| Content Type | Text |
