isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase (1997)

Content Provider	CiteSeerX
Author	Kinoshita, Yasunori Nishigaki, Koichi Husimi, Yuzuru
Abstract	A rapid 5′-labeling method of single-stranded DNA/ RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5′-ends of both oligo- and polynucleotides. End labeling of DNA/RNA is an essential technique for nucleic acids research. Enzymic methods for end-labeling tend to be easier and more convenient than organic chemical methods due to advantages such as mildness, specificity, controllability and efficiency of enzymic reactions. Enzymic 3′-labeling techniques already exist: TdT methods and T4 RNA ligase methods can add a modified nucleotide to DNA/RNA at its 3′-end (1–4). However, there exists no simple enzymic 5′-end labeling technique which
File Format	PDF
Journal	Nucleic Acids Res
Language	English
Publisher Date	1997-01-01
Access Restriction	Open
Subject Keyword	T4 Rna Ligase Single-stranded Dna Rna Enzymic Reaction Nucleic Acid Research Modified Nucleotide T4 Rna Ligase Method Organic Chemical Method End Labeling Essential Technique End Labeling Technique Enzymic Method Dna Rna Adenylated Intermediate End-labeling Tend Tdt Method
Content Type	Text
Resource Type	Article