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Novel bacterial and archaeal lineages from an in situ growth chamber deployed at a Mid-Atlantic Ridge hydrothermal vent (2000)
| Content Provider | CiteSeerX |
|---|---|
| Author | Reysenbach, Anna-Louise Longnecker, Krista Kirshtein, Julie |
| Abstract | The phylogenetic diversity was determined for a microbial community obtained from an in situ growth chamber placed on a deep-sea hydrothermal vent on the Mid-Atlantic Ridge (23°22 � N, 44°57 � W). The chamber was deployed for 5 days, and the temperature within the chamber gradually decreased from 70 to 20°C. Upon retrieval of the chamber, the DNA was extracted and the small-subunit rRNA genes (16S rDNA) were amplified by PCR using primers specific for the Archaea or Bacteria domain and cloned. Unique rDNA sequences were identified by restriction fragment length polymorphisms, and 38 different archaeal and bacterial phylotypes were identified from the 85 clones screened. The majority of the archaeal sequences were affiliated with the Thermococcales (71%) and Archaeoglobales (22%) orders. A sequence belonging to the Thermoplasmales confirms that thermoacidophiles may have escaped enrichment culturing attempts of deep-sea hydrothermal vent samples. Additional sequences that represented deeply rooted lineages in the low-temperature eurarchaeal (marine group II) and crenarchaeal clades were obtained. The majority of the bacterial sequences obtained were restricted to the Aquificales (18%), the � subclass of the Proteobacteria (�-Proteobacteria) (40%), and the genus Desulfurobacterium (25%). Most of the clones (28%) were confined to a monophyletic clade within the �-Proteobacteria with no known close relatives. The prevalence of clones related to thermophilic microbes that use hydrogen as an electron donor and sulfur compounds (S 0,SO 4, thiosulfate) indicates the importance of |
| File Format | |
| Journal | Appl Environ Microbiol |
| Language | English |
| Publisher Date | 2000-01-01 |
| Access Restriction | Open |
| Subject Keyword | Situ Growth Chamber Mid-atlantic Ridge Hydrothermal Vent Archaeal Lineage Genus Desulfurobacterium Phylogenetic Diversity Additional Sequence Crenarchaeal Clade Bacterial Sequence Mid-atlantic Ridge Unique Rdna Sequence Deep-sea Hydrothermal Vent Low-temperature Eurarchaeal Different Archaeal Monophyletic Clade Electron Donor Archaeal Sequence Sequence Belonging Restriction Fragment Length Polymorphism Microbial Community Small-subunit Rrna Gene Bacterial Phylotypes Bacteria Domain Marine Group Ii Sulfur Compound Deep-sea Hydrothermal Vent Sample |
| Content Type | Text |
| Resource Type | Article |
