NDLI: The role of cytotoxic t lymphocytes in corneal allograft rejection (2000).

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The role of cytotoxic t lymphocytes in corneal allograft rejection (2000).

Content Provider	CiteSeerX
Author	Hegde, Sushma Niederkorn, Jerry Y.
Abstract	PURPOSE. Immunologic rejection constitutes a major barrier to the success of allogeneic corneal transplants, but the specific mediators and mechanisms of graft rejection are poorly understood. Several studies have implicated cytotoxic T-lymphocyte (CTL) responses, typically associated with CD8 � T cells, in promoting corneal graft rejection. This study sought to test the hypothesis that CTLs are essential in promoting corneal graft rejection. METHODS. BALB/c donor corneas were grafted orthotopically onto C57BL/6, perforin knockout, or CD8 � T-cell knockout mice. The tempo and incidence of graft rejection were observed for each group. In separate experiments, donor-specific CTL and delayed-type hypersensitivity (DTH) responses were tested at the time of graft rejection by a standard chromium release assay and an ear swelling assay, respectively. RESULTS. Perforin knockout and CD8 � T-cell knockout mice were as effective as wild-type C57BL/6 control mice in rejecting BALB/c donor corneas. Furthermore, animals in all three groups were found to develop robust donor-specific DTH, not CTL, responses at the time of graft rejection. Histopathologically, the rejected corneas from all three groups contained a predominantly mononuclear cellular infiltrate.
File Format	PDF
Publisher Date	2000-01-01
Access Restriction	Open
Subject Keyword	Graft Rejection Corneal Allograft Rejection Corneal Graft Rejection Perforin Knockout Cd8 T-cell Knockout Mouse Allogeneic Corneal Transplant Cd8 Cell Balb Donor Cornea Donor-specific Ctl Separate Experiment Immunologic Rejection Mononuclear Cellular Infiltrate Specific Mediator Cytotoxic T-lymphocyte Standard Chromium Release Assay Delayed-type Hypersensitivity Donor Cornea Control Mouse Major Barrier Several Study Wild-type C57bl Robust Donor-specific Dth
Content Type	Text

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