NDLI: Experimental study comparison of leptin gene expression in different adipose tissues in children and adults.

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Experimental study comparison of leptin gene expression in different adipose tissues in children and adults.

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Abstract	Objective: Adipose tissue displays depot-specific metabolic properties and a predominant gene expression of leptin in subcutaneous tissue. The aim of the study was to evaluate leptin mRNA expression in various adipose tissues and to relate it to plasma leptin concentrations. Furthermore, developmental changes in leptin gene expression from childhood to adulthood were examined. Design and methods: Thoracic subcutaneous and intrathoracic adipose tissue specimens were obtained in 22 adults (51–81 years) and 23 children (0.1 –17 years) undergoing cardiac surgery, and abdominal subcutaneous, omental and mesenterial fat specimens were collected from 21 adults (38 –79 years) and 22 children (0.2 –17 years) before abdominal surgery. Preoperative plasma leptin concentrations were measured by RIA. Leptin mRNA expression was quantified by TaqMan real-time PCR. Results: In adults, there was no difference between leptin gene expression in subcutaneous and intrathoracic fat, whereas in children leptin mRNA expression was significantly higher in subcutaneous adipose tissue. In omental fat, leptin mRNA levels were significantly lower compared with subcutaneous and mesenterial sites in both children and adults. Adults revealed a significantly higher leptin gene expression in subcutaneous, omental and mesenterial adipose tissues than children.
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Subject Keyword	Leptin Gene Expression Different Adipose Tissue Experimental Study Comparison Leptin Mrna Expression Intrathoracic Fat Various Adipose Tissue Mesenterial Adipose Tissue Subcutaneous Tissue Omental Fat Subcutaneous Adipose Tissue Mesenterial Site Leptin Concentration Intrathoracic Adipose Tissue Specimen Preoperative Plasma Leptin Concentration Depot-specific Metabolic Property Cardiac Surgery Adipose Tissue Developmental Change Predominant Gene Expression Taqman Real-time Pcr Abdominal Surgery Mesenterial Fat Specimen Child Leptin Mrna Expression Mrna Level
Content Type	Text

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