NDLI: Mammalian Stem Cells Reprogramming in Response to Terahertz Radiation (2010)

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Mammalian Stem Cells Reprogramming in Response to Terahertz Radiation (2010)

Content Provider	CiteSeerX
Author	Fukuyo, Yayoi Martinez, Jennifer S. Alex, Boian S. Bock, Jonathan Rosen, Evan D. Kim Ø Chen, Hou-Tong Kang, Sona Alex, Ludmil B. Usheva, Anny Bishop, Alan R.
Abstract	We report that extended exposure to broad-spectrum terahertz radiation results in specific changes in cellular functions that are closely related to DNA-directed gene transcription. Our gene chip survey of gene expression shows that whereas 89 % of the protein coding genes in mouse stem cells do not respond to the applied terahertz radiation, certain genes are activated, while other are repressed. RT-PCR experiments with selected gene probes corresponding to transcripts in the three groups of genes detail the gene specific effect. The response was not only gene specific but also irradiation conditions dependent. Our findings suggest that the applied terahertz irradiation accelerates cell differentiation toward adipose phenotype by activating the transcription factor peroxisome proliferator-activated receptor gamma (PPARG). Finally, our molecular dynamics computer simulations indicate that the local breathing dynamics of the PPARG promoter DNA coincides with the gene specific response to the THz radiation. We propose that THz radiation is a potential tool for cellular
File Format	PDF
Publisher Date	2010-01-01
Access Restriction	Open
Subject Keyword	Molecular Dynamic Computer Simulation Gene Expression Cellular Function Gene Specific Response Gene Probe Pparg Promoter Dna Dna-directed Gene Transcription Mouse Stem Cell Gene Chip Survey Irradiation Condition Dependent Potential Tool Broad-spectrum Terahertz Radiation Result Applied Terahertz Irradiation Gene Specific Effect Terahertz Radiation Mammalian Stem Cell Reprogramming Local Breathing Dynamic Specific Change Certain Gene Thz Radiation Rt-pcr Experiment Adipose Phenotype
Content Type	Text

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