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RESEARCH Open Access Alternative splicing of the Anopheles gambiae Dscam gene in diverse Plasmodium falciparum
| Content Provider | CiteSeerX |
|---|---|
| Author | Obbard, Darren J. Yan, Guiyun Ranford-Cartwright, Lisa C. Afrane, Yaw A. Little, Tom J. Smith, Paul H. Mwangi, Jonathan M. |
| Abstract | Background: In insects, including Anopheles mosquitoes, Dscam (Down syndrome cell adhesion molecule) appears to be involved in phagocytosis of pathogens, and shows pathogen-specific splice-form expression between divergent pathogen (or parasite) types (e.g. between bacteria and Plasmodium or between Plasmodium berghei and Plasmodium falciparum). Here, data are presented from the first study of Dscam expression in response to genetic diversity within a parasite species. Methods: In independent field and laboratory studies, a measure of Dscam splice-form diversity was compared between mosquitoes fed on blood that was free of P. falciparum to mosquitoes exposed to either single or mixed genotype infections of P. falciparum. Results: Significant increases in Anopheles gambiae Dscam (AgDscam) receptor diversity were observed in parasiteexposed mosquitoes, but only weak evidence that AgDscam diversity rises further upon exposure to mixed genotype parasite infections was found. Finally, a cluster of AgDscam exon 4 variants that become especially common during Plasmodium invasion was identified. Conclusions: While the data clearly indicate that AgDscam diversity increases with P. falciparum exposure, they do not suggest that AgDscam diversity rises further in response to increased parasite diversity. |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Agdscam Diversity Laboratory Study Mixed Genotype Infection Dscam Splice-form Diversity Parasite Specie Parasiteexposed Mosquito Weak Evidence Anopheles Gambiae Dscam Genetic Diversity Dscam Expression Pathogen-specific Splice-form Expression Falciparum Exposure Receptor Diversity Diverse Plasmodium Falciparum Mixed Genotype Parasite Infection Plasmodium Falciparum Anopheles Mosquito Research Open Access Alternative Splicing Adhesion Molecule Agdscam Diversity Increase Plasmodium Berghei Plasmodium Invasion Anopheles Gambiae Dscam Gene Significant Increase Parasite Diversity Independent Field Divergent Pathogen |
| Content Type | Text |
