NDLI: Effects of the histone deacetylase inhibitor valproic acid on human pericytes in vitro (2011).

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Effects of the histone deacetylase inhibitor valproic acid on human pericytes in vitro (2011).

Content Provider	CiteSeerX
Author	Karén, Jakob Rodriguez, Ro Friman, Tomas Dencker, Lennart Sundberg, Christian Scholz, Birger
Abstract	Microvascular pericytes are of key importance in neoformation of blood vessels, in stabilization of newly formed vessels as well as maintenance of angiostasis in resting tissues. Furthermore, pericytes are capable of differentiating into pro-fibrotic collagen type I producing fibroblasts. The present study investigates the effects of the histone deacetylase (HDAC) inhibitor valproic acid (VPA) on pericyte proliferation, cell viability, migration and differentiation. The results show that HDAC inhibition through exposure of pericytes to VPA in vitro causes the inhibition of pericyte proliferation and migration with no effect on cell viability. Pericyte exposure to the potent HDAC inhibitor Trichostatin A caused similar effects on pericyte proliferation, migration and cell viability. HDAC inhibition also inhibited pericyte differentiation into collagen type I producing fibroblasts. Given the importance of pericytes in blood vessel biology a qPCR array focusing on the expression of mRNAs coding for proteins that regulate angiogenesis was performed. The results showed that HDAC inhibition promoted transcription of genes involved in vessel stabilization/maturation in human microvascular pericytes. The present in vitro study demonstrates that VPA influences several aspects of microvascular pericyte biology and suggests an alternative mechanism by which HDAC inhibition affects blood vessels. The results raise the possibility that HDAC inhibition inhibits
File Format	PDF
Publisher Date	2011-01-01
Access Restriction	Open
Subject Keyword	Hdac Inhibition Histone Deacetylase Inhibitor Valproic Acid Human Pericytes Pericyte Proliferation Cell Viability Blood Vessel Human Microvascular Pericytes Hdac Inhibition Inhibits Histone Deacetylase Potent Hdac Inhibitor Trichostatin Similar Effect Microvascular Pericytes Pericyte Differentiation Qpcr Array Alternative Mechanism Hdac Inhibition Affect Blood Vessel Inhibitor Valproic Acid Pro-fibrotic Collagen Type Pericyte Exposure Vessel Stabilization Maturation Blood Vessel Biology Collagen Type Vitro Study Microvascular Pericyte Biology Present Study Several Aspect Key Importance
Content Type	Text

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