NDLI: RESEARCH Open Access Modeling CICR in rat ventricular myocytes: voltage clamp studies

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RESEARCH Open Access Modeling CICR in rat ventricular myocytes: voltage clamp studies

Content Provider	CiteSeerX
Author	Clark, John W. Krishna, Abhilash Sun, Liang Valderrábano, Miguel Palade, Philip T.
Abstract	Background: The past thirty-five years have seen an intense search for the molecular mechanisms underlying calcium-induced calcium-release (CICR) in cardiac myocytes, with voltage clamp (VC) studies being the leading tool employed. Several VC protocols including lowering of extracellular calcium to affect Ca 2+ loading of the sarcoplasmic reticulum (SR), and administration of blockers caffeine and thapsigargin have been utilized to probe the phenomena surrounding SR Ca 2+ release. Here, we develop a deterministic mathematical model of a rat ventricular myocyte under VC conditions, to better understand mechanisms underlying the response of an isolated cell to calcium perturbation. Motivation for the study was to pinpoint key control variables influencing CICR and examine the role of CICR in the context of a physiological control system regulating cytosolic Ca 2+ concentration ([Ca 2+]myo). Methods: The cell model consists of an electrical-equivalent model for the cell membrane and a fluid-compartment model describing the flux of ionic species between the extracellular and several intracellular compartments (cell cytosol, SR and
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Access Restriction	Open
Subject Keyword	Cytosolic Ca Vc Condition Cell Membrane Voltage Clamp Study Deterministic Mathematical Model Cell Cytosol Sarcoplasmic Reticulum Cell Model Intense Search Cardiac Myocytes Extracellular Calcium Calcium-induced Calcium-release Rat Ventricular Myocyte Key Control Variable Ionic Specie Rat Ventricular Myocytes Voltage Clamp Physiological Control System Molecular Mechanism Several Vc Protocol Isolated Cell Research Open Access Modeling Cicr Several Intracellular Compartment Sr Ca Fluid-compartment Model Past Thirty-five Year Electrical-equivalent Model
Content Type	Text

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