NDLI: Human placental cytotrophoblasts attract monocytes and cd56 bright natural killer cells via the actions of monocyte inflammatory protein 1.

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Human placental cytotrophoblasts attract monocytes and cd56 bright natural killer cells via the actions of monocyte inflammatory protein 1.

Content Provider	CiteSeerX
Author	Tsou, Chia-Lin Zhou, Yan Huang, Ling Fisher, Susan J.
Abstract	During human pregnancy, the specialized epithelial cells of the placenta (cytotrophoblasts) come into direct contact with immune cells in several locations. In the fetal compartment of the placenta, cytotrophoblast stem cells lie adjacent to macrophages (Hofbauer cells) that reside within the chorionic villus stroma. At sites of placental attachment to the mother, invasive cytotrophoblasts encounter specialized maternal natural killer (NK) cells (CD56bright), macrophages, and T cells that accumulate within the uterine wall during pregnancy. Here we tested the hypothesis that fetal cytotrophoblasts can direct the migration of these maternal immune cells. First, we assayed the chemotactic activity of cytotrophoblast conditioned medium samples, using human peripheral blood mononuclear cells as targets. The placental samples preferentially attracted NK cells (both CD56dim and CD56bright), monocytes, and T cells, suggesting that our hypothesis was correct. A screen to identify chemokine activity through the induction of a Ca2 � flux in cells transfected with individual chemokine receptors suggested that cytotrophoblasts secreted monocyte inflammatory protein (MIP)-1�. This was confirmed by localizing the corresponding mRNA and protein, both in vitro and in vivo. MIP-1 � protein in conditioned medium was
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Subject Keyword	Monocyte Inflammatory Protein Human Placental Cytotrophoblasts Attract Monocyte Natural Killer Cell Fetal Compartment Ca2 Flux Medium Sample Individual Chemokine Receptor Human Peripheral Blood Mononuclear Cell Chemotactic Activity Chorionic Villus Stroma Direct Contact Placental Sample Cytotrophoblast Stem Cell Placental Attachment Conditioned Medium Invasive Cytotrophoblasts Fetal Cytotrophoblasts Corresponding Mrna Mip-1 Protein Maternal Natural Killer Several Location Immune Cell Hofbauer Cell Chemokine Activity Nk Cell Uterine Wall Maternal Immune Cell Specialized Epithelial Cell Human Pregnancy
Content Type	Text

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