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Applied microbial and cell physiology degradation of 4-fluorophenol by arthrobacter sp. Strain if1.
| Content Provider | CiteSeerX |
|---|---|
| Author | Isabel, Maria Ferreira, M. Marchesi, Julian R. Janssen, Dick B. Marchesi, J. R. |
| Abstract | Abstract A Gram-positive bacterial strain capable of aerobic biodegradation of 4-fluorophenol (4-FP) as the sole source of carbon and energy was isolated by selective enrichment from soil samples collected near an industrial site. The organism, designated strain IF1, was identified as a member of the genus Arthrobacter on the basis of 16S ribosomal RNA gene sequence analysis. Arthrobacter strain IF1 was able to mineralize 4-FP up to concentrations of 5 mM in batch culture. Stoichiometric release of fluoride ions was observed, suggesting that there is no formation of halogenated dead-end products during 4-FP metabolism. The degradative pathway of 4-FP was investigated using enzyme assays and identification of intermediates by gas chromatography (GC), GC–mass spectrometry (MS), highperformance liquid chromatography, and liquid chromatography–MS. Cell-free extracts of 4-FP-grown cells contained no activity for catechol 1,2-dioxygenase or catechol 2,3dioxygenase, which indicates that the pathway does not proceed through a catechol intermediate. Cells grown on 4-FP oxidized 4-FP, hydroquinone, and hydroxyquinol but not 4-fluorocatechol. During 4-FP metabolism, hydroquinone accumulated as a product. Hydroquinone could be converted to hydroxyquinol, which was further transformed into maleylacetic acid and β-ketoadipic acid. These results |
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| Access Restriction | Open |
| Subject Keyword | Strain If1 Applied Microbial Cell Physiology Degradation Arthrobacter Sp 4-fp Metabolism Gram-positive Bacterial Strain Industrial Site Dead-end Product Cell-free Extract Fluoride Ion Highperformance Liquid Chromatography Liquid Chromatography M Sole Source Genus Arthrobacter Degradative Pathway Soil Sample 4-fp-grown Cell Batch Culture Aerobic Biodegradation Stoichiometric Release Gc Mass Spectrometry Ketoadipic Acid Enzyme Assay Gas Chromatography Catechol Intermediate Selective Enrichment Maleylacetic Acid Ribosomal Rna Gene Sequence Analysis Arthrobacter Strain If1 |
| Content Type | Text |
