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GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro (1992)
| Content Provider | CiteSeerX |
|---|---|
| Author | Boman, Annettel Delannoy, T. Michael R. Wilson, Katherine L. |
| Abstract | Abstract. Nuclear envelope assembly was studied in vitro using extracts from Xenopus eggs. Nuclearspecific vesicles bound to demembranated sperm chromatin but did not fuse in the absence of cytosol. Addition of cytosol stimulated vesicle fusion, pore complex assembly, and eventual nuclear envelope growth. Vesicle binding and fusion were assayed by light and electron microscopy. Addition of ATP and GTP to bound vesicles caused limited vesicle fusion, but enclosure of the chromatin was not observed. This result suggested that nondialyzable soluble components were required for nuclear vesicle fusion. GTPyS and guanylyl imidodiphosphate significantly inhibited vesicle fusion but had no effect on vesicle binding to chromatin. Preincubation of membranes with 1 mM |
| File Format | |
| Journal | J. Cell |
| Language | English |
| Publisher Date | 1992-01-01 |
| Access Restriction | Open |
| Subject Keyword | Vesicle Fusion Nuclear Envelope Assembly Gtp Hydrolysis Vesicle Binding Eventual Nuclear Envelope Growth Guanylyl Imidodiphosphate Nuclear Vesicle Fusion Pore Complex Assembly Sperm Chromatin Nondialyzable Soluble Component Xenopus Egg Electron Microscopy Limited Vesicle Fusion |
| Content Type | Text |
| Resource Type | Article |
