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Reactivation of ethanol-calcium-isolated from tetrahymena pyriformis cilia.
| Content Provider | CiteSeerX |
|---|---|
| Abstract | Cilia isolated from Tetrahymena after ciliary shedding is induced by the addition of CaC1 2 or other salts to cells suspended in an ethanol solution (4, 6, 11) have been studied chemically and enzymatically (4, 6, 1i, 12, 13), but until now have never been shown to be capable of motility in the presence of ATP. In contrast to this, Gibbons (7) has demonstrated the reactivation of glycerolisolated cilia by techniques similar to those developed for grasshopper sperm tails (8) and Polytoma flagella (2). However, the glycerol technique is much less satisfactory than the ethanolcalcium method for isolating pure cilia. This paper describes the reactivation, which can be obtained with ATP or ADP, of cilia isolated |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Ethanol-calcium-isolated Tetrahymena Pyriformis Cilium Ethanol Solution Ciliary Shedding Grasshopper Sperm Tail Polytoma Flagellum Glycerol Technique Ethanolcalcium Method Pure Cilium |
| Content Type | Text |
| Resource Type | Article |
