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Simple sequence repeats provide a substrate for phenotypic variation in the neurospora crassa circadian clock.
| Content Provider | CiteSeerX |
|---|---|
| Author | Michael, Todd P. Park, Sohyun Kim, Tae-Sung Booth, Jim Byer, A. Sun, Qi Chory, Joanne Lee, Kwangwon |
| Abstract | Background. WHITE COLLAR-1 (WC-1) mediates interactions between the circadian clock and the environment by acting as both a core clock component and as a blue light photoreceptor in Neurospora crassa. Loss of the amino-terminal polyglutamine (NpolyQ) domain in WC-1 results in an arrhythmic circadian clock; this data is consistent with this simple sequence repeat (SSR) being essential for clock function. Methodology/Principal Findings. Since SSRs are often polymorphic in length across natural populations, we reasoned that investigating natural variation of the WC-1 NpolyQ may provide insight into its role in the circadian clock. We observed significant phenotypic variation in the period, phase and temperature compensation of circadian regulated asexual conidiation across 143 N. crassa accessions. In addition to the NpolyQ, we identified two other simple sequence repeats in WC-1. The sizes of all three WC-1 SSRs correlated with polymorphisms in other clock genes, latitude and circadian period length. Furthermore, in a cross between two N. crassa accessions, the WC-1 NpolyQ co-segregated with period length. Conclusions/Significance. Natural variation of the WC-1 NpolyQ suggests a mechanism by which period length can be varied and selected for by the local environment that does not deleteriously affect WC-1 activity. Understanding natural variation in the N. crassa circadian clock will facilitate an understanding of how fungi exploit their |
| File Format | |
| Access Restriction | Open |
| Subject Keyword | Simple Sequence Repeat Provide Wc-1 Npolyq Phenotypic Variation Natural Variation Neurospora Crassa Circadian Clock Simple Sequence Repeat Circadian Clock Crassa Accession Period Length Asexual Conidiation Blue Light Photoreceptor Crassa Circadian Clock Wc-1 Result Local Environment Natural Population Clock Gene Wc-1 Ssrs Significant Phenotypic Variation Methodology Principal Finding Amino-terminal Polyglutamine Affect Wc-1 Activity Neurospora Crassa Circadian Period Length Conclusion Significance Arrhythmic Circadian Clock Temperature Compensation Core Clock Component Clock Function |
| Content Type | Text |
