Crude extracts of caenorhabditis elegans suppress airway inflammation in a murine model of allergic asthma.

Content Provider	CiteSeerX
Author	Kim, Sung Eun Kim, Jae-Hwan Min, Byung-Hoon Bae, Young Mee Hong, Sung-Tae Choi, Min-Ho
Abstract	Epidemiological studies suggest an inverse relationship between helminth infections and allergic disease, and several helminth-derived products have been shown to suppress allergic responses in animals. This study was undertaken to evaluate the effect of a crude extract of Caenorhabditis elegans on allergic airway inflammation in a murine model of asthma. Allergic airway inflammation was induced in BALB/c mice by sensitization with ovalbumin. The effect of the C. elegans crude extract on the development of asthma and on established asthma was evaluated by analyzing airway hyperresponsiveness, serum antibody titers, lung histology and cell counts and cytokine levels in the bronchoalveolar lavage fluid. The role of IFN-c in the suppression of asthma by the C. elegans crude extract was investigated in IFN-c knockout and wild-type mice. When mice were sensitized with ovalbumin together with the crude extract of C. elegans, cellular infiltration into the lung was dramatically reduced in comparison with the ovalbumin-treated group. Treatment of mice with the C. elegans crude extract significantly decreased methacholine-induced airway hyperresponsiveness and the total cell counts and levels of IL-4, IL-5 and IL-13 in the bronchoalveolar lavage fluid but increased the levels of IFN-c and IL-12. Sensitization with the C. elegans crude extract significantly diminished the IgE and IgG1 responses but provoked elevated IgG2a levels. However, the suppressive effect of the C. elegans crude extract was abolished in IFN-c knockout mice, and the Th2 responses in these mice were as strong as those in wild-type mice sensitized with ovalbumin. The crude extract
File Format	PDF
Access Restriction	Open
Subject Keyword	Crude Extract Murine Model Allergic Asthma Suppress Airway Inflammation Allergic Airway Inflammation Wild-type Mouse Bronchoalveolar Lavage Fluid Helminth Infection Epidemiological Study Methacholine-induced Airway Hyperresponsiveness Ovalbumin-treated Group Inverse Relationship Several Helminth-derived Product Total Cell Count Igg1 Response Ifn-c Knockout Cellular Infiltration Allergic Response Th2 Response Cytokine Level Balb Mouse Lung Histology Caenorhabditis Elegans Allergic Disease Ifn-c Knockout Mouse Cell Count Serum Antibody Titer Igg2a Level Suppressive Effect
Content Type	Text