NDLI: The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium

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The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium

Content Provider	bioRxiv
Author	Willoughby, Patrick Morley Allen, Molly Yu, Jessica Korytnikov, Roman Chen, Tianhui Liu, Yupeng So, Isis Macpherson, Neil Mitchell, Jennifer Fernandez-gonzalez, Rodrigo Ashley, E. E. Bruce
Copyright Year	2021
Abstract	Abstract In emerging epithelial tissues, cells undergo dramatic rearrangements to promote tissue shape changes. Dividing cells remain interconnected via transient cytokinetic bridges. Bridges are cleaved during abscission and currently, the consequences of disrupting abscission in developing epithelia are not well understood. We show that the Rab GTPase, Rab25, localizes near cytokinetic midbodies and likely coordinates abscission through endomembrane trafficking in the epithelium of the zebrafish gastrula during epiboly. In maternal-zygotic Rab25a and Rab25b mutant embryos, morphogenic activity tears open persistent apical cytokinetic bridges that failed to undergo timely abscission. Cytokinesis defects result in anisotropic cell morphologies that are associated with a reduction of contractile actomyosin networks. This slows cell rearrangements and alters the viscoelastic responses of the tissue, all of which likely contribute to delayed epiboly. We present a model in which Rab25 trafficking coordinates cytokinetic bridge abscission and cortical actin density, impacting local cell shape changes and tissue-scale forces.
Related Links	https://www.biorxiv.org/content/biorxiv/early/2021/01/29/2021.01.29.428759.full.pdf
DOI	10.1101/2021.01.29.428759
Language	English
Publisher	Cold Spring Harbor Laboratory
Publisher Date	2021-01-29
Access Restriction	Open
Rights License	Creative Commons License (Attribution 4.0 International), CC BY 4.0
Subject Keyword	Cell Biology
Content Type	Text
Resource Type	Preprint
Subject	Cell Biology

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