NDLI: SARS-CoV-2 epitope mapping on microarrays highlights strong immune-response to N protein region

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SARS-CoV-2 epitope mapping on microarrays highlights strong immune-response to N protein region

Content Provider	bioRxiv
Author	Musicò, Angelo Frigerio, Roberto Mussida, Alessandro Barzon, Luisa Sinigaglia, Alessandro Riccetti, Silvia Gobbi, Federico Piubelli, Chiara Bergamaschi, Greta Chiari, Marcella Gori, Alessandro Cretich, Marina
Copyright Year	2020
Abstract	Abstract A workflow for SARS-CoV-2 epitope discovery on peptide microarrays is herein reported. The process started with a proteome-wide screening of immunoreactivity based on the use of a high-density microarray followed by a refinement and validation phase on a restricted panel of probes using microarrays with tailored peptide immobilization through a click-based strategy. Progressively larger, independent cohorts of Covid-19 positive sera were tested in the refinement processes, leading to the identification of immunodominant regions on SARS-CoV-2 Spike (S), Nucleocapsid (N) protein and Orf1ab polyprotein. A summary study testing 50 serum samples highlighted an epitope of the N protein (region 155-171) providing 92% sensitivity and 100% specificity of IgG detection in Covid-19 samples thus being a promising candidate for rapid implementation in serological tests.
Related Links	https://www.biorxiv.org/content/biorxiv/early/2020/11/09/2020.11.09.374082.full.pdf
DOI	10.1101/2020.11.09.374082
Language	English
Publisher	Cold Spring Harbor Laboratory
Publisher Date	2020-11-09
Access Restriction	Open
Subject Keyword	Biochemistry
Content Type	Text
Resource Type	Preprint
Subject	Biochemistry

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