NDLI: Grazing incidence x-ray diffraction studies of lipid-peptide mixed monolayers during shear ﬂow

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Grazing incidence x-ray diffraction studies of lipid-peptide mixed monolayers during shear ﬂow

Content Provider	Azim Premji Foundation
Author	Sanyal, Milan K. Imp´eror-Clerc, Marianne Krishnaswamy, Rema Maiti, Santanu Sood, A. K. Pansu, Brigitte Kandar, Ajoy Kumar Constantin, Doru Bera, Pradip Kumar
Abstract	Grazing Incidence X-ray Diﬀraction (GIXD) studies of monolayers of biomolecules atthe air-water interface give quantitative information of in-plane packing, coherence lengths ofthe ordered (diﬀracting) domains and orientation of hydrocarbon chains of the model membranes. Rheo-GIXD measurements revel quantitative changes in the monolayer under shear.Here we report GIXD studies of monolayers of Alamethicin peptide, DPPC lipid and theirmixtures at the air-water interface under the application of steady shear stresses. The Alamethicin monolayer and the mixed monolayer show ﬂow jamming transition. On the other hand,pure DPPC monolayer under the constant stress ﬂows steadily with a notable enhancement of1arXiv:1908.00228v1 [cond-mat.soft] 1 Aug 2019area/molecule, coherence length, and the tilt angle with increasing stress, suggesting fusion ofdomains during ﬂow. The DPPC-Alamethicin mixed monolayer shows no signiﬁcant changein the area/DPPC molecule or in the DPPC chain tilt angle but the coherence length of bothphases (DPPC and Alamethicin) increases suggesting that the domains of individual phasesaremergingtobiggersizepromotingmoreseparationofphasesinthesystemduringﬂow. Ourresults show that Rheo-GIXD has the potential to explore in-situ molecular structural changesunder rheological conditions for a diverse range of conﬁned biomolecules at the interfaces.
Alternate Webpage(s)	https://publications.azimpremjiuniversity.edu.in/2049/1/Grazing%20incidence%20x-ray%20diffraction%20studies%20of%20lipid-peptide%20mixed%20monolayers%20during%20shear%20%EF%AC%82ow.pdf
Language	English
Publisher	Cornell University
Publisher Date	2019-08-01
Access Restriction	Open
Subject Keyword	DPPC-Alamethicin X-ray Alamethicin Grazing Incidence X-ray Diﬀraction (GIXD) DPPC
Content Type	Text
Resource Type	Article

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