NDLI: Interactions Between Circulating Tumor Cells and Aptamer-Functionalized Microposts in a Flow

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Chen, Kangfu Georgiev, Teodor Fan, Z. Hugh
Copyright Year	2017
Abstract	Circulating Tumor Cells (CTCs) have been considered as important biomarkers for cancer prognosis and treatment. However, there are only tens of CTCs in one billion of healthy blood cells. This CTC rarity challenge has been addressed by microfluidics technology that sheds light on efficient CTC detection and isolation. Using antibodies or aptamers to capture CTCs is one of the strategies for CTC isolation. A lot of work has been carried out to improve CTC capture efficiency and purity (i.e., specificity). The main consideration to optimize microfluidic device performance includes increasing surface-area-to-volume ratio and reducing shear stress, both of which are closely related to the interaction between CTCs and the microfluidic device. Here we report a detailed study on the interactions between CTCs and aptamer-functionalized microposts in a microfluidic device. We have evaluated the distribution of captured CTCs around a micropost. In addition, simulation was conducted to model CTC capture patterns around microposts. We found the simulated CTC capture pattern largely agree with the experimental results. The simulation methodology could be applicable for other affinity-based CTC isolation devices and approaches. The goal of the study is to improve the microfluidic device performance and provide a rapid and economical way to optimize the geometry design of the microfluidic devices for CTC isolation.
File Format	PDF
ISBN	9780791858424
DOI	10.1115/IMECE2017-70342
Volume Number	Volume 7: Fluids Engineering
Conference Proceedings	ASME 2017 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2017-11-03
Publisher Place	Tampa, Florida, USA
Access Restriction	Subscribed
Subject Keyword	Design Geometry Simulation Shear stress Flow (dynamics) Microfluidics Blood Tumors Cancer
Content Type	Text
Resource Type	Article

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