NDLI: Experimental Demonstration and Numerical Simulation of Organic-Aqueous Liquid Extraction Enhanced by Droplet Formation in a Microfluidic Channel

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Mao, Xiaole Yang, Sung Jeffrey, D. Zahn
Copyright Year	2006
Abstract	Organic-aqueous (phenol) liquid extraction is one of the commonly used DNA purification methods. Effective mass transfer of biological material between the discrete fluid phases is key to achieving efficient extraction when designing microfluidic devices based on this technique. In the microscale regime, mass transfer is often diffusion limited. However, mass transfer can be enhanced through the formation of discrete droplets within a microchannel, which leads to a recirculation flow pattern within the droplet. This recirculation increases the mass transfer rate of material to the organic-aqueous interface. In this study, an experimental and computational examination of sample extraction between the organic and aqueous phases through droplet formation is presented. The experiment is conducted within a converging dual inlet microfluidic channel fabricated in PDMS. By controlling the capillary number of the flow, different flow patterns are created in the channel. The flow patterns are examined using a computational fluid dynamics (CFD) simulation. The CFD model successfully simulates the flow behavior under a variety of flow conditions and provides a closer examination of the internal recirculation pattern within the droplet. The experimental sample extraction utilizes a fluorescent dye localization technique and shows that the droplet flow offers a significant improvement in the speed of sample extraction over diffusional mixing. A preliminary test demonstrates the feasibility of using the droplet formation for fast extraction with biological samples.
Sponsorship	Fluids Engineering Division
Starting Page	213
Ending Page	220
Page Count	8
File Format	PDF
ISBN	0791847705
DOI	10.1115/IMECE2006-16084
e-ISBN	0791837904
Volume Number	Fluids Engineering
Conference Proceedings	ASME 2006 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2006-11-05
Publisher Place	Chicago, Illinois, USA
Access Restriction	Subscribed
Subject Keyword	Computer simulation Computational fluid dynamics Microscale devices Plasma desorption mass spectrometry Flow (dynamics) Diffusion (physics) Mass transfer Microchannels Design Fluids Simulation Dna Drops Microfluidics
Content Type	Text
Resource Type	Article

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