NDLI: Multiscale Mechanical Evaluation of Human Supraspinatus Tendon Under Shear Loading After Glycosaminoglycan Reduction

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Fang, Fei Lake, Spencer P.
Copyright Year	2017
Abstract	Proteoglycans (PGs) are broadly distributed within many soft tissues and, among other roles, often contribute to mechanical properties. Although PGs, consisting of a core protein and glycosaminoglycan (GAG) sidechains, were once hypothesized to regulate stress/strain transfer between collagen fibrils and help support load in tendon, several studies have reported no changes to tensile mechanics after GAG depletion. Since GAGs are known to help sustain nontensile loading in other tissues, we hypothesized that GAGs might help support shear loading in human supraspinatus tendon (SST), a commonly injured tendon which functions in a complex multiaxial loading environment. Therefore, the objective of this study was to determine whether GAGs contribute to the response of SST to shear, specifically in terms of multiscale mechanical properties and mechanisms of microscale matrix deformation. Results showed that chondroitinase ABC (ChABC) treatment digested GAGs in SST while not disrupting collagen fibers. Peak and equilibrium shear stresses decreased only slightly after ChABC treatment and were not significantly different from pretreatment values. Reduced stress ratios were computed and shown to be slightly greater after ChABC treatment compared to phosphate-buffered saline (PBS) incubation without enzyme, suggesting that these relatively small changes in stress values were not due strictly to tissue swelling. Microscale deformations were also not different after ChABC treatment. This study demonstrates that GAGs possibly play a minor role in contributing to the mechanical behavior of SST in shear, but are not a key tissue constituent to regulate shear mechanics.
Starting Page	071013
Ending Page	071018
Page Count	6
File Format	PDF
ISSN	01480731
e-ISSN	15288951
Journal	Journal of Biomechanical Engineering
Volume Number	139
Issue Number	7
DOI	10.1115/1.4036602
Language	English
Publisher	The American Society of Mechanical Engineers
Publisher Date	2017-06-06
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biological tissues Biomechanics Deformation Enzymes Equilibrium (Physics) Fibers Mechanical behavior Mechanical properties Microscale devices Shear (Mechanics) Stress Tendons
Content Type	Text
Resource Type	Article
Subject	Physiology (medical) Biomedical Engineering

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