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| Content Provider | African Journals Online (AJOL) |
|---|---|
| Author | MA Sheikh T Iftikhar S Rasul MA Zia |
| Abstract | Along with other emerging health consequences, diabetes mellitus is one of the critical threats spreading all over the world with continuity. The investigation of glucose level in diabetic patients requires the key enzyme β-D-glucose:oxygen-1-oxidoreductase/glucose oxidase, where its optimized production by mutant derived strain could affect the economic burden and accuracy of the test. Wild type Aspergillus niger was subjected to ultraviolet radiation for enhanced production of glucose oxidase. It was found that UV-180-C is a potential mutant derived strain, screened by using 2-deoxy-Dglucose. Optimum production of glucose oxidase from A. niger UV-180-C was carried out by using CSL (2%), fermentation period (36 h), pH (5.5 and 4.5 for wild and mutant respectively), temperature (30°C), MgSO4.7H2O (0.0%), CaCO3 (0.1%), KH2PO4 (0.8 and 1.0% for wild and mutant strains), Urea (0.3%). Crude enzyme was subjected to ammonium sulfate precipitation and resulted into 145.8 UmL-1 activity. Glucose oxidase from mutant derived A. niger exhibited optimum pH at 6, temperature 20°C, Km 10mM and Vmax 142 UmL-1. The pyridoxal phosphate caused significant inhibition to the enzyme which indicates the presence of lysyl residues near or at the active site of the enzyme from both wild and mutant derived strains.Key words:Enhanced production, UV radiations, β-D-glucose:oxygen-1-oxidoreductase, A. niger |
| Ending Page | 14533 |
| Starting Page | 14522 |
| File Format | |
| e-ISSN | 16845315 |
| DOI | 10.5897/AJB11.1477 |
| Journal | African Journal of Biotechnology |
| Issue Number | 65 |
| Volume Number | 10 |
| Language | English |
| Publisher Date | 2013-11-18 |
| Access Restriction | Open |
| Subject Keyword | Biology Life Sciences Enhanced production UV radiations β-D-glucose oxygen-1-oxidoreductase A. niger |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biotechnology Molecular Biology Agronomy and Crop Science Genetics Applied Microbiology and Biotechnology |
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