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| Content Provider | African Journals Online (AJOL) |
|---|---|
| Author | W Liu X Lan M Chen Z Liao W Wang C Yang |
| Abstract | Geranylgeranyl pyrophosphate synthase (GGDPS, EC: 2.5.1.29) catalyzes the biosynthesis of 20-carbon geranylgeranyl pyrophosphate (GGDP), which is a key precursor for ginkgolides biosynthesis.In order to investigate the role of GGDP synthase in ginkgolides biosynthesis, we cloned, characterized and functionally expressed the GGDP synthase gene from Ginkgo biloba. The genomic DNA fragmentof the GGDPS gene of G. biloba (designated GbGGDPS GenBank accession number EF646377) was 2135 bp in length containing an 1176-bp open reading frame (ORF) that encoded a 391-amino acidpolypeptide. Comparative analysis showed that GbGGDPS had a high similarity to other plant GGDPSs. Bioinformatic analysis showed that GbGGDPS was an intron-free gene and its deduced polypeptidecontained all the five conserved domains and functional aspartate-rich motifs of the polyprenyltransferases. By constructing the phylogenetic tree of plant GGDPSs, it was found that plant-derived GGDPSs could be divided into two classes, angiosperm and gymnosperm classes, whichmight have evolved in parallel from the same ancestor. The homology-based structural modelingshowed that GbGGDPS has the typical structure of GGDPS. The tissue expression profiles ofGbGGDPS indicated that it could express in roots, stems, leaves, peshes and fruits but at differentlevels. The highest expression level of GbGGDPS was found in roots and peshes while the lowestexpression level of GbGGDPS was found in leaves. Interestingly, it was found that GbGGDPS had ahigher expression level in the treatment of methyl jasmonate. Finally, the coding sequence ofGbGGDPS was functionally expressed in Escherica coli in which the -carotene pathway wasreconstructed by genetic complementation, and the transgenic E. coli showed to have an activity ofGGDP synthase. |
| File Format | |
| e-ISSN | 16845315 |
| Journal | African Journal of Biotechnology |
| Issue Number | 7 |
| Volume Number | 8 |
| Language | English |
| Publisher Date | 2010-09-27 |
| Access Restriction | Open |
| Subject Keyword | Biology Life Sciences Ginkgo biloba geranylgeranyl pyrophosphate synthase cloning characterization expression profile functional complementation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biotechnology Molecular Biology Agronomy and Crop Science Genetics Applied Microbiology and Biotechnology |
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