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| Content Provider | African Journals Online (AJOL) |
|---|---|
| Author | TK Mukiama LG Matasyoh AWT Muigai MG Kinyua FN Wachira JC Matasyoh |
| Abstract | Hydro-distilled volatile oils from the leaves of Ocimum gratissimum L. (Lamiaceae) of 13 populations of different silvicultural zones were evaluated for antimicrobial activity against Gram positive (Staphylococcus aereus, Bacillus spp.) and Gram negative (Escherichia coli, Pseudomonas aeruginosa, Samonella typhi, Klebisiella pneumoniae, Proteus mirabilis) bacteria and a pathogenic fungus, Candida albicans. All the essential oils are active to the tested microbiles with different strength. The highest antimicrobial activity against Gram positive bacteria (Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosae and Proteus mirabilis) was observed from the eastern Kenya (Meru) oil. Meru oil was the best and its effectiveness was consistent on nearly all the microbes tested. The oil from the plant growing in the coastal region of Kenya (Mombasa) showed the best effect only on Gram negative bacteria (Escherichia coli and Proteus mirabilis). Both oils (Meru and Mombasa) were dominated by monoterpenes accounting for 92.48 % and 81.37 % respectively. The monoterpene fraction was characterized by a high percentage of eugenol (68.8 %) for Meru oil and 74.10 % for Mombasa oil. The other major monoterpene was methyl eugenol (13.21 %). Camphor (0.95 %) was observed only in the Meru oil. (Cis)-Ocimene, (trans)-ocimene and β-pinene were present in both Meru and Mombasa oils. The sesquiterpenes present in fairly good amounts in both oils were germacrene D and (trans)-caryophyllene. The minor sesquiterpenes were α- farnesene (0.85 %) and β-bisabolene (0.74 %) which were present in the Meru oil only. |
| Ending Page | 193 |
| Starting Page | 187 |
| File Format | |
| e-ISSN | 01896016 |
| DOI | 10.4314/ajtcam.v5i2.31272 |
| Journal | African Journal of Traditional, Complementary and Alternative Medicines |
| Issue Number | 2 |
| Volume Number | 5 |
| Language | English |
| Publisher Date | 2008-10-17 |
| Access Restriction | Open |
| Subject Keyword | Chemistry Mathematics Physics African Studies Health Sociology Anthropology Antimicrobial activity Ocimum gratissimum L. Eugenol Essential oil |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Complementary and Alternative Medicine |
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