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| Content Provider | African Journals Online (AJOL) |
|---|---|
| Author | J Zhou |
| Abstract | Alliinase was immobilized on chitosan microspheres by means of glutaraldehyde, and a flow-injection enzymatic analytical system was developed for determination of alliin based on the immobilizedalliinase and an ammonia gas electrode. The factors affecting the activity of the immobilized enzyme, such as glutaraldehyde concentration, cross-linking time and the amount of alliinase, were investigated. Results showed that the maximum of enzyme activity could be obtained at glutaraldehyde concentration of 4%, cross-linking time of 2 h and the amount of alliinase 20.2 u. The properties of the immobilized alliinase were also studied in detail. For the immobilized alliinase, the highest activity was allowed at pH of 7.0 and temperature at 35°C. Besides, the immobilized enzyme showed good thermal and pH stabilities. The flow-injection enzymatic analytical system based on the immobilized alliinase and an ammonia gas electrode provided linearity in the 1 × 10-5 to 1 × 10-3 mol/L alliin concentrationrange and exhibited good repeatability and operational stability. |
| File Format | |
| e-ISSN | 16845315 |
| Journal | African Journal of Biotechnology |
| Issue Number | 7 |
| Volume Number | 8 |
| Language | English |
| Publisher Date | 2010-09-27 |
| Access Restriction | Open |
| Subject Keyword | Biology Life Sciences Alliinase immobilization enzyme properties flow-injection analysis. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biotechnology Molecular Biology Agronomy and Crop Science Genetics Applied Microbiology and Biotechnology |
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