NDLI: Phytochemistry and antioxidant assays of Entandrophragma angolense (Welw.) C.DC. (meliaceae) using DPPH and nitric oxide free radical scavenging methods

Phytochemistry and antioxidant assays of Entandrophragma angolense (Welw.) C.DC. (meliaceae) using DPPH and nitric oxide free radical scavenging methods

Content Provider African Journals Online (AJOL)
Author A.A. Oyawaluja J.S. Babasanmi O Soneye B.O. Oyawaluja
Abstract Background: Medicinal plants have always been a veritable source of bioactive compounds and also serve as ‘lead molecules’ and templates for the design of medicinally useful synthetic molecules. Entandrophragma angolense Meliaceae, chemically characterized by synthesis of modified triterpenes known as limonoids which are powerful antioxidants and may help to prevent certain cancers and cardiovascular disease.Objectives: To carry out phytochemical screening of the methanol bark and leaf extracts of Entandrophragma angolense, evaluate and compare the antioxidant activity of the methanol bark and leaf extracts of Entandrophragma angolense.Method: The different phytochemicals in E. angolense were screened for using standard methods. The antioxidant activity of the plant was accessed using two DPPH free radical scavenging and Nitric oxide scavenging activities.Result: The presence of alkaloids, flavonoids, condensed and hydrolysable tannins, saponins, steroidal nucleus, anthraquinones and reducing sugars in the methanolic bark extract were confirmed, with similar result for the methanolic leaf extract, except for the absence of alkaloids, anthraquinone and reducing sugars. The antioxidant assays indicated that both extracts from the two morphological plant parts showed considerable antioxidant activities (bark IC50 0.45, leaf IC50 0.41 for DPPH assay and bark IC50 0.42, leaf IC50 0.38 μg/ml for Nitric oxide assay) when compared with the standards (Ascorbic acid IC50 0.40 and IC50 0.40 μg/ml for DPPH and Nitric oxide assay, respectively, and Gallic acid, IC50 0.25 and IC50 0.35 μg/ml for DPPH and Nitric oxide assay, respectively).Conclusion: E. angolense could be a potential source of natural antioxidant that could have greater importance as therapeutic agent in preventing or slowing accumulation of lipid peroxidation, and thereby oxidative stress related degenerative diseases.
Ending Page 235
Starting Page 229
File Format PDF
ISSN 01898434
e-ISSN 26353555
DOI 10.4314/njpr.v15i2.10
Journal Nigerian Journal of Pharmaceutical Research
Issue Number 2
Volume Number 15
Language English
Publisher Date 2020-01-30
Access Restriction Open
Subject Keyword Health Phytochemical screening Entandrophragma angolense Antioxidant DPPH Nitric oxide
Content Type Text
Resource Type Article
Subject Medicine

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Phytochemistry and antioxidant assays of Entandrophragma angolense (Welw.) C.DC. (meliaceae) using DPPH and nitric oxide free radical scavenging methods

Content Provider	African Journals Online (AJOL)
Author	A.A. Oyawaluja J.S. Babasanmi O Soneye B.O. Oyawaluja
Abstract	Background: Medicinal plants have always been a veritable source of bioactive compounds and also serve as ‘lead molecules’ and templates for the design of medicinally useful synthetic molecules. Entandrophragma angolense Meliaceae, chemically characterized by synthesis of modified triterpenes known as limonoids which are powerful antioxidants and may help to prevent certain cancers and cardiovascular disease.Objectives: To carry out phytochemical screening of the methanol bark and leaf extracts of Entandrophragma angolense, evaluate and compare the antioxidant activity of the methanol bark and leaf extracts of Entandrophragma angolense.Method: The different phytochemicals in E. angolense were screened for using standard methods. The antioxidant activity of the plant was accessed using two DPPH free radical scavenging and Nitric oxide scavenging activities.Result: The presence of alkaloids, flavonoids, condensed and hydrolysable tannins, saponins, steroidal nucleus, anthraquinones and reducing sugars in the methanolic bark extract were confirmed, with similar result for the methanolic leaf extract, except for the absence of alkaloids, anthraquinone and reducing sugars. The antioxidant assays indicated that both extracts from the two morphological plant parts showed considerable antioxidant activities (bark IC50 0.45, leaf IC50 0.41 for DPPH assay and bark IC50 0.42, leaf IC50 0.38 μg/ml for Nitric oxide assay) when compared with the standards (Ascorbic acid IC50 0.40 and IC50 0.40 μg/ml for DPPH and Nitric oxide assay, respectively, and Gallic acid, IC50 0.25 and IC50 0.35 μg/ml for DPPH and Nitric oxide assay, respectively).Conclusion: E. angolense could be a potential source of natural antioxidant that could have greater importance as therapeutic agent in preventing or slowing accumulation of lipid peroxidation, and thereby oxidative stress related degenerative diseases.
Ending Page	235
Starting Page	229
File Format	PDF
ISSN	01898434
e-ISSN	26353555
DOI	10.4314/njpr.v15i2.10
Journal	Nigerian Journal of Pharmaceutical Research
Issue Number	2
Volume Number	15
Language	English
Publisher Date	2020-01-30
Access Restriction	Open
Subject Keyword	Health Phytochemical screening Entandrophragma angolense Antioxidant DPPH Nitric oxide
Content Type	Text
Resource Type	Article
Subject	Medicine

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