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| Content Provider | African Journals Online (AJOL) |
|---|---|
| Author | Xavier Worowounga Clotaire Donatien Rafai Jean-Laurent Syssa-Magalé Ernest Lango-Yaya Armel-Frederic Namkona Mathurin Issa-Madongo Marinette Saravolia |
| Abstract | Objectif : La résistance aux antibiotiques des souches bactériennes pose depuis quelques décennies un problème de santé publique dans le monde, car les antibiotiques de la dernière génération qui avaient un effet bactériostatique et bactéricide sur les germes deviennent de plus en plus inefficaces. C’est dans ce contexte que cette étude a été réalisée pour évaluer la sensibilité des extraits méthanolique des écorces de Paullinia pinnata et des feuilles de Petaclethra macrophylla sur les souches de Staphylococcus aureus, Shigella dysenteriae et Salmonella paratyphi A en République Centrafricaine. Méthodologie et Résultats : Pour ce travail, macération a été réalisée sous agitation pendant quatre heures pour obtenir les extraits méthanoliques de ces deux plantes. Les techniques de diffusion et de dilution ont été utilisées pour déterminer les effets de principes actifs de P. macrophylla et de P. pinnata sur les souches de S. aureus, de S. paratyphi A et de S. dysenteriae. Le rendement de l’extraction des écorces de P. pinnata a été de 3,75/20 (18,76%) et celui des feuilles de P. macrophylla étaient de 1,60/20 (8,00%). Les extraits de deux plantes ont un effet bactériostatique sur la souche de S. aureus avec des diamètresd’inhibition respective de 25,00±0,50 et de 27,00±0,50 mm ce qui n’était pas significativement différent du diamètre de la référence, le chloramphénicol (29,00±0,00 mm). Par contre, les extraits de P. pinnata et de P. macrophylla ont inhibé de manière sélective les deux souches S. dysenteriae et S. paratyphi A. Les concentrations minimales inhibitrices (CMI) des extraits varient entre 0,05 et 0,09mg/mL et celles des concentrations minimales bactéricides (CMB) entre 0,05 et 0,14mg/mL. Conclusion : Les deux plantes évaluées dans cette étude sont bien connues pour le traitement de quelques infections liées aux bactéries dans les différentes régions de la République Centrafricaine (RCA). Selon les résultats de cette étude, les principes actifs des extraits de ces deux plantes ont des effets bactericides et bactériostatiques. Il est nécessaire d’approfondir les études afin de leur exploitation et leur utilisation dans le traitement des infections bactériennes en RCA. English abstract Objective: Resistance of bacterial strains to antibiotics has posed a public health problem in the world in the past decades, since the last generation of antibiotic, which has a bacteriostatic and bactericidal effect, are becoming increasingly ineffective. It is in this context that this study was carried out to assess the sensitivity of the methanolic extract of Paullinia pinnata root barks and Petaclethra macrophylla leaves on Staphylococcus aureus , Shigella dysenteriae et Salmonella paratyphi A in Central African Republic. Methodology and Results: Maceration was done under stirring for four hours to obtain the methanolic extract of the two plants; diffusion and dilution in liquid method were used to determine the effects of the active ingredients of P. macrophylla and P. pinnata on the strains of S. aureus, S. dysenteriae and S. paratyphi A. The extraction yield of P. pinnata root barks of 3.75/20 (18.76%) is higher than the P. macrophylla leaves, which is 1.60/20 (8.00%). The minimum inhibitory concentrations (MIC) of extracts varied between 0.05 and 0.09 mg/mL and the minimum bactericidal concentrations (MBC) was between 0.05 and 0.14 mg/mL. The extracts of the two plants induced bacteriostatic activity against S. aureus and inhibited its growth at 25.00±0.50 and 27.00±0.50 mm diameter, respectively for P. pinnata and P.macrophylla, which was not significantly different from the chloramphenicol reference at 29.00±0.00 mm. The extracts of P. pinnata and P. macrophylla selectively inhibited S. dysenteriae and S. paratyphi A. Conclusion: The two plants evaluated in this study are well known for their use in the treatment of bacterial infections in the different regions of Central African Republic. According to the results of this study, the active ingredients of the extracts of the two plants have bactericidal and bacteriostatic y effects on bacteria. It is necessary to conduct further studies towards their exploitation and use in treating bacterial infections in Central African Republic. Key words: Bacterial activity, methanolic extract, Paullinia pinnata and Petraclethra macrophylla. |
| Ending Page | 15158 |
| Starting Page | 15151 |
| File Format | |
| e-ISSN | 19975902 |
| Journal | Journal of Applied Biosciences |
| Issue Number | 1 |
| Volume Number | 147 |
| Language | English French |
| Publisher Date | 2022-10-11 |
| Access Restriction | Open |
| Subject Keyword | Chemistry Mathematics Physics Biology Life Sciences General Science Activité antibactérienne extrait méthanolique Paullinia pinnata Petaclethra macrophylla |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Biotechnology Molecular Biology Decision Sciences |
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