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Protein structure-based method for identifying horizontal gene transfer

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Protein structure-based method for identifying horizontal gene transfer

Content Provider	ACM Digital Library
Author	Griep, Mark A. Revesz, Peter Z. Santosh, Venkat R. B.
Abstract	Genetics has traditionally focused on vertical gene transfer, which is the passing of the genetic material of an organism to its offspring. However, recent studies in genetics increased the awareness that horizontal gene transfer, which is the passing of the genetic material of an organism to another organism that is not its offspring, is also a significant phenomenon. Horizontal gene transfer is thought to play a major role in the natural evolution of bacteria, such as, when several different types of bacteria all suddenly develop the same drug resistance genes. Artificial horizontal gene transfer occurs in genetic engineering. This paper provides methods to detect horizontal gene transfer among bacteria using BLAST and DaliLite measures of protein sequence and structural similarities. This research is novel and unique because no previous horizontal gene transfer study worked directly on protein sequences and structures. The main method is a computer algorithm to detect horizontal gene transfer among different COG classifications of proteins. The paper also considers visual structural comparisons and sequence alignments using the 'Jmol' tool. Finally, the paper considers the possibility that the methods yield false positives.
Starting Page	9
Ending Page	16
Page Count	8
File Format	PDF
ISBN	9781450306263
DOI	10.1145/1992896.1992898
Language	English
Publisher	Association for Computing Machinery (ACM)
Publisher Date	2011-05-16
Publisher Place	New York
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article

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