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Techniques for Scanned Illumination
| Content Provider | The Lens |
|---|---|
| Related Links | https://www.lens.org/images/patent/EP/2859396/B1/EP_2859396_B1.pdf |
| Language | English |
| Publisher Date | 2021-07-21 |
| Access Restriction | Open |
| Alternative Title | Verfahren Für Abgetastete Beleuchtung Techniques D'éclairage À Balayage |
| Content Type | Text |
| Resource Type | Patent |
| Date Applied | 2013-06-05 |
| Agent | Eisenführ Speiser |
| Applicant | Complete Genomics Inc |
| Application No. | 13801133 |
| Claim | A method for operating an imaging system (100), the method comprising: collecting a two-dimensional image of a portion of a substrate (105) on a positioning stage (110) via a digital camera (125) in the imaging system (100) by moving the positioning stage (110) in a first path of travel under an objective lens component (115) of the imaging system (100) that is normal to the optical axis of the objective lens component (115), wherein the image spans multiple rows of pixels of a sensor element in the camera (125); while the camera (125) is collecting the image of the portion of the substrate (105), performing steps comprising: changing an angle of a scan mirror (130) that is disposed in the optical path between the objective lens component (115) and the camera (125) so that the image of the portion of the substrate (105) is kept still with respect to the camera (125) while the positioning stage is moving; scanning a line of light generated by a line generator (160) across the portion of the substrate (105) by changing an angle of an illumination mirror (140), thereby exposing first one or more rows of pixels spanned by the image while keeping in the dark second one or more rows of pixels spanned by the image; and reading out the first one or more rows of pixels while continuing to scan the line of light across the portion of the substrate (105) thereby exposing the second one or more rows of pixels. The method of Claim 1, wherein changing the angle of the scan mirror (130) is performed in coordination with scanning the line of light across the portion of the substrate (105). The method of Claim 1, wherein: changing the angle of the scan mirror (130) includes a first time interval during which the line of light is scanned across the portion of the substrate (105) and a second time interval during which the scan mirror (130) is returned to an initial position; and the method further comprises ceasing to scan the line of light across the portion of the substrate (105) during the second time interva The method of Claim 3, wherein the first time interval is greater than the second time interva The method of Claim 1, wherein reading out the first one or more rows of pixels is performed while the substrate (105) is being moved in a direction that is normal to the optical axis of the objective lens component (115). The method of Claim 1, wherein changing the angle of the illumination mirror (140) is effected by a servo mechanism configured to tilt the illumination mirror (140). The method of Claim 1, further comprising keeping the first one or more rows of pixels dark between the steps of exposing the first one or more rows of pixels and reading out the first one or more rows of pixels. The method of Claim 1, wherein the camera (125) comprises a split-readout sensor having at least a first section and a second section, and the steps of scanning the line of light and reading out are performed independently in each section of the split-readout sensor. The method of Claim 8, wherein the steps of scanning and reading out in the first section are performed in parallel to the steps of scanning and reading out in the second section. The method of Claim 8, wherein the steps of scanning and reading out in the first section are performed in anti-parallel to the steps of scanning and reading out in the second section. The method of Claim 1, further comprising: while the camera (125) is collecting the image of the portion of the substrate (105), reading out the second one or more rows of pixels while continuing to scan the line of light across the substrate (105) thereby exposing third one or more rows of pixels spanned by the image, wherein the third one or more rows of pixels are different than the second one or more rows of pixels. The method of Claim 1, wherein the camera (125) is one of a CMOS camera and a non-CMOS camera operating in full-frame mode. The method of Claim 11, wherein the third one or more rows of pixels are different than the first one or more rows of pixels. The method of Claim 1, further comprising flying back the scan mirror while continuing to read from the sensor element. An imaging system (100) comprising: an objective lens component (115); a line generator (160) that is configured to generate a line of light that is scanned across a portion of a substrate (105); a positioning stage (110) that is configured to move the substrate (105) in a first path of travel that is substantially normal to an optical axis of the objective lens component (115); a digital camera (125) that is configured to acquire a two-dimensional image of the portion of the substrate (105) through the objective lens component (115); a scan mirror (130) that is configured to move in coordination with the positioning stage (110), while the line of light is being scanned across the portion of the substrate (105) and the substrate (105) is being moved in the first path of travel in a continuous motion, in order to maintain the image still with respect to the camera (125) while the image is being acquired by the camera (125); and an illumination mirror (140) and a computer logic, wherein the image spans multiple rows of pixels in the camera (125), and wherein while the camera (125) is acquiring the image of the portion of the substrate (105): the illumination mirror (140) is configured and operative to scan the line of light across the portion of the substrate (105) to expose first one or more rows of pixels spanned by the image while keeping in the dark second one or more rows of pixels spanned by the image; and the computer logic is configured and operative to read out the first one or more rows of pixels while the illumination mirror (140) continues to scan the line of light across the portion of the substrate (105) to expose the second one or more rows of pixels. The imaging system (100) of Claim 15, further comprising an illumination mirror (140) configured to scan the line of light from the line generator onto the portion of the substrate (105), wherein the scan mirror (130) is configured to move in coordination with the illumination mirror (140) while the line of light is being scanned across the portion of the substrate (105) and the substrate (105) is being moved in the particular direction. The imaging system (100) of Claim 16, further comprising: a first servo mechanism configured to change an angle of the illumination mirror (140) to scan the line of light across the portion of the substrate (105); and a second servo mechanism configured to change angle of the scan mirror (130) in coordination with changes to the angle of the illumination mirror (140). The imaging system (100) of Claim 17, wherein: the second servo mechanism is configured to change angle of the scan mirror (130) during a first time interval in which the line of light is scanned across the portion of the substrate (105); the second servo mechanism is configured to return the scan mirror (130) to an initial position during a second time interval in which the line of light is not scanned across the portion of the substrate (105), wherein the second time interval is shorter than the first time interva The imaging system (100) of Claim 15, further comprising a dichroic mirror that is configured at least to: (a) reflect the line of light from the illumination mirror (140) onto the scan mirror (130) in order to illuminate the portion of the substrate (105); and (b) pass to the camera (125) light acquired by the objective lens component (115) and is reflected by the scan mirror (130). The imaging system (100) of Claim 15, wherein the camera (125) comprises a split-readout sensor having at least two sections that can be exposed and read out independently of each other. The imaging system (100) of Claim 15, wherein the system is configured to fly back the scan mirror while continuing to read from the sensor element. |
| CPC Classification | Investigating Or Analysing Materials By Determining Their Chemical Or Physical Properties OPTICAL ELEMENTS; SYSTEMS OR APPARATUS IMAGE DATA PROCESSING OR GENERATION; IN GENERAL PICTORIAL COMMUNICATION; e.g. TELEVISION |
| Extended Family | 015-024-383-036-933 189-799-366-297-57X 174-357-489-102-178 106-772-070-827-024 026-110-924-606-004 147-914-489-297-614 183-411-277-705-824 102-033-300-895-491 171-076-044-387-043 167-391-455-583-001 032-144-611-569-508 197-907-996-039-028 174-332-014-218-803 051-069-711-225-464 085-010-086-025-738 044-318-619-511-047 189-391-695-802-195 125-003-229-592-099 092-836-709-902-050 161-172-245-053-135 193-650-615-371-953 110-064-039-416-566 049-943-677-648-303 120-717-883-958-203 129-383-615-753-973 |
| Patent ID | 2859396 |
| Inventor/Author | Staker Bryan P Uhrich Craig E |
| IPC | G02B21/00 G02B21/36 G06K9/58 |
| Status | Active |
| Owner | Complete Genomics, Inc. |
| Simple Family | 015-024-383-036-933 085-010-086-025-738 044-318-619-511-047 189-799-366-297-57X 106-772-070-827-024 125-003-229-592-099 183-411-277-705-824 092-836-709-902-050 193-650-615-371-953 032-144-611-569-508 110-064-039-416-566 120-717-883-958-203 129-383-615-753-973 |
| CPC (with Group) | G01N21/6456 G02B21/365 G06T1/0007 G06T2207/30072 G06T2207/10056 G01N21/6452 H04N25/78 H04N3/08 H04N23/56 H04N25/441 H04N25/531 H04N25/767 H04N25/75 G02B21/361 H04N7/18 G06T2207/10064 G06T2207/10152 G06T2207/30004 |
| Issuing Authority | European Patent Office (EPO) |
| Kind | Patent/Patent 1st level of publication/Inventor's certificate |
