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Micro-fluidic Device and Uses Thereof
| Content Provider | The Lens |
|---|---|
| Abstract | A micro-fluidic device includes at least one inlet and a curvilinear microchannel having a trapezoidal cross section defined by a radially inner side, a radially outer side, a bottom side, and a top side, the cross section having a) the radially inner side and the radially outer side unequal in height, or b) the radially inner side equal in height to the radially outer side, and wherein the top side has at least two continuous straight sections, each unequal in width to the bottom side. |
| Related Links | https://www.lens.org/lens/patent/013-091-258-275-211/frontpage |
| Language | English |
| Publisher Date | 2017-10-17 |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Patent |
| Jurisdiction | United States of America |
| Date Applied | 2013-09-20 |
| Agent | Hamilton, Brook, Smith & Reynolds. P.c. |
| Applicant | Massachusetts Inst Technology Nat Univ Singapore |
| Application No. | 201314429280 |
| Claim | A micro-fluidic device that can preferentially generate at least one strong Dean vortex along a flow stream for at least one of particle trapping and cell trapping, the micro-fluidic device comprising: i) at least one inlet; ii) a curvilinear microchannel having a cross section defined by a constant width along the microchannel, and a radially inner side, a radially outer side, a bottom side, and a top side, the cross section having the radially inner side and the radially outer side unequal in height, resulting in the generation of at least one strong Dean vortex core, a channel depth of the radially outer side being between about 100 μm and about 140 μm, and a channel depth of the radially inner side being between about 70 μm and about 90 μm; the curvilinear microchannel being configured to trap at least one of a particle and a cell in the at least one strong Dean vortex along the radially outer side of the curvilinear microchannel; and iii) at least one outlet. The micro-fluidic device of claim 1 , wherein the top side includes at least one step forming a stepped profile. The micro-fluidic device of claim 1 , wherein the cross section is a right trapezoidal cross section. The micro-fluidic device of claim 1 , wherein the top side or bottom side of the cross section is curved. The micro-fluidic device of claim 4 , wherein the top side or the bottom side of the cross section is convex or concave. The micro-fluidic device of claim 1 , wherein the top side or bottom side of the cross section has a width in a range of between about 100 microns and about 2000 microns. The micro-fluidic device of claim 1 , wherein the curvilinear microchannel is a spiral microchannel or a serpentine microchanne The micro-fluidic device of claim 1 , wherein the curvilinear microchannel has a radius of curvature in a range of between about 2.5 mm and about 25 mm. The micro-fluidic device of claim 1 , wherein the curvilinear microchannel has a length in a range of between about 4 cm and about 100 cm. The micro-fluidic device of claim 1 , wherein the cross section is a right trapezoidal cross section comprising a slant angle of between about 2 degrees and about 60 degrees. A method of separating by size one or more particles from a mixture of particles suspended in a liquid media, comprising introducing the mixture into at least one inlet of a micro-fluidic device that can preferentially generate at least one strong Dean vortex along a flow stream for particle trapping, the micro-fluidic device comprising: i) at least one inlet; ii) a curvilinear microchannel having a cross section defined by a constant width along the microchannel, and a radially inner side, a radially outer side, a bottom side, and a top side, the cross section having the height of the radially inner side being smaller than the height of the radially outer side, resulting in the generation of at least one strong Dean vortex core, a channel depth of the radially outer side being between about 100 μm and about 140 μm, and a channel depth of the radially inner side being between about 70 μm and about 90 μm; the curvilinear microchannel being configured to trap at least one of a particle and a cell in the at least one strong Dean vortex along the radially outer side of the curvilinear microchannel; and iii) at least one outlet; the mixture being introduced into the at least one inlet of the micro-fluidic device at a flow rate that isolates particles along portions of the cross-section of the microchannel based on particle size, wherein larger particles flow along the radially inner side of the microchannel to a first outlet and smaller particles are trapped in at least one strong Dean vortex core near the radially outer side and flow to at least one other outlet, thereby size separating the particles from the mixture. The method of claim 11 , wherein the particles are cells or a mixture of cells. The method of claim 12 , wherein the cells are stem cells; the mixture of cells is a blood sample; the mixture of cells is a bone marrow sample; the mixture of cells are stem cells and hematologic cells; the mixture of cells are CTC's and hematologic cells; or the mixture of cells are leukocytes and hematologic cells. A method of mixing cells in a liquid, comprising introducing a liquid and cells into at least one inlet of a micro-fluidic device, the micro-fluidic device comprising: i) at least one inlet; ii) a curvilinear microchannel having a cross section defined by a constant width along the microchannel, and a radially inner side, a radially outer side, a bottom side, and a top side, the cross section having the radially inner side equal in height to the radially outer side, and wherein the top side has at least two continuous straight sections, each unequal in width to the bottom side, the top side including at least one shallow region, between the radially inner side and the radially outer side, that is shallower than both a channel depth of the radially inner side and a channel depth of the radially outer side; and iii) at least one outlet; the introducing the liquid and the cells into the at least one inlet of the micro-fluidic device being at a flow rate that mixes cells along the microchannel and directs the mixture to a first outlet and optionally collecting the mixture from the first outlet. A micro-fluidic device for mixing cells in a liquid, the micro-fluidic device comprising: i) at least one inlet; ii) a curvilinear microchannel having a cross section defined by a constant width along the microchannel, and a radially inner side, a radially outer side, a bottom side, and a top side, the cross section having the radially inner side equal in height to the radially outer side, wherein the top side has at least two continuous straight sections, each unequal in width to the bottom side, and wherein the top side includes at least one shallow region, between the radially inner side and the radially outer side, that is shallower than both a channel depth of the radially inner side and a channel depth of the radially outer side; and iii) at least one outlet. |
| CPC Classification | Chemical Or Physical Laboratory Apparatus For General Use Separation APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY;APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS; FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS; i.e. BIOREACTORS OR FERMENTERS Mixing; E.G. Dissolving; Emulsifying Or Dispersing SEPARATING SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS |
| Examiner | Paul Hyun |
| Extended Family | 150-130-134-495-702 184-575-759-379-205 130-641-093-887-257 093-877-237-267-761 104-558-429-326-34X 028-762-398-175-369 123-795-561-193-167 031-043-442-480-182 083-077-590-674-589 031-726-845-789-670 093-309-220-068-904 082-395-961-555-464 090-914-446-737-53X 013-091-258-275-211 |
| Patent ID | 9789485 |
| Inventor/Author | Han Jongyoon Chen Chao Yu Peter Ong Chong Jin Guan Guofeng Bhagat Ali Asgar Wu Lidan |
| IPC | B01D21/26 B01D21/00 B01F5/06 B01L3/00 B01L99/00 B03B5/62 |
| Status | Active |
| Owner | Massachusetts Institute of Technology National University of Singapore |
| Simple Family | 150-130-134-495-702 184-575-759-379-205 130-641-093-887-257 093-877-237-267-761 104-558-429-326-34X 028-762-398-175-369 123-795-561-193-167 031-043-442-480-182 083-077-590-674-589 031-726-845-789-670 093-309-220-068-904 082-395-961-555-464 090-914-446-737-53X 013-091-258-275-211 |
| CPC (with Group) | B01L3/502753 B01D21/0087 B01D21/265 B01L2200/0652 B01L2300/0816 B01L2300/0864 B01L2400/0478 B01L2400/0487 B01L2400/084 C12M47/04 B01D21/00 B01F25/4231 B01L3/50273 B01L2300/0883 B01L2400/0463 B03B5/62 |
| Issuing Authority | United States Patent and Trademark Office (USPTO) |
| Kind | Patent/New European patent specification (amended specification after opposition procedure) |
