3d Polymeric Insert to Apply Uniform Electric Field in Circular Cultureware

Content Provider	The Lens
Related Links	https://www.lens.org/lens/patent/012-540-342-599-336/frontpage
Language	English
Publisher Date	2019-05-22
Access Restriction	Open
Alternative Title	3d-polymereinsatz Zum Anlegen Eines Gleichmässigen Elektrischen Feldes in Kreisförmigen Kulturgefässen Élément Inséré Polymère Tridimensionnel Pour L'application D'un Champ Électrique Uniforme Dans Un Matériel De Culture Circulaire
Content Type	Text
Resource Type	Patent
Date Applied	2016-09-16
Agent	Haseltine Lake Llp
Applicant	Okinawa Inst Science & Tech School Corp
Application No.	16845972
Claim	An insert to be inserted into a circular-shaped petri dish for generating a substantially uniform electric field across the petri dish filled with a fluid establishing a salt bridge, comprising: a circular-shaped bottom plate configured to be fitted into the circular-shaped petri dish, the bottom plate defining a circular-shaped space of a substantially uniform thickness together with a circular-shaped bottom surface of the petri dish as a channel accepting said fluid when the insert is inserted into the petri dish; a side channel vertically erecting from a circular periphery of said bottom plate, the side channel communicating with said circular shaped space defined by the bottom plate so as to accept said fluid; and a pair of current rectifying chambers each having a generally planar shape connecting to and communicating with the side channel, the current rectifying chambers being diagonally disposed to face each other along a diameter of the circular shaped bottom plate and being line-symmetric with each other with respect to said diameter, each of the current rectifying chambers extending inwardly from a respective portion of a top end of the side channel and having a salt bridge port to accept the fluid and provide the fluid to said circular-shaped space defined by the bottom plate through said side channel so as to establish the salt bridge, wherein portions of the side channel other than the portions thereof respectively connecting to the current rectifying chambers each have a generally concave top profile having a lowest point at a center between the pair of current rectifying chambers and are each shaped such that when the salt bridge is established, the circular-shaped space defined by the bottom plate exhibits a substantially uniform electric field in a direction of said diameter in a substantially entire area thereof. The insert according to claim 1, wherein the circular shaped petri dish is a tissue-culture polystyrene (TCPS) petri dish. The insert according to claim 1, wherein said generally concave top profile of the side channel outlines a curve defined by an intersection of a paraboloid with a cylindrical column. The insert according to claim 3, wherein said generally concave top profile of the side channel has a stair-like shape outlining said curve. A device for establishing a uniform electric field in a circular-shaped space filled with said fluid having a prescribed resistivity, comprising a unitary or multipart member that comprises the insert according to any one of claims 1-4 and the circular-shaped petri dish.
CPC Classification	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY;APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS; FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS; i.e. BIOREACTORS OR FERMENTERS
Extended Family	122-990-908-967-457 004-925-244-726-466 160-375-253-362-230 012-540-342-599-336 175-755-905-374-026 170-733-780-797-308 020-193-670-359-776
Patent ID	3350310
Inventor/Author	Tsai Hsieh-fu Fried Amy Shen Cheng Ji-yen
IPC	C12M3/00 C12M1/00
Status	Active
Simple Family	122-990-908-967-457 004-925-244-726-466 160-375-253-362-230 012-540-342-599-336 175-755-905-374-026 170-733-780-797-308 020-193-670-359-776
CPC (with Group)	C12M35/02 C12M23/02 C12M23/10
Issuing Authority	United States Patent and Trademark Office (USPTO)
Kind	Patent/Patent 1st level of publication/Inventor's certificate